Effect of oil contaminants on antioxidant responses and antioxidant properties of Pleurotus florida (P. Kumm)

ABSTRACT

This research investigated the antioxidant responses of Pleurotus florida at different concentrations of gas oil [0% (control), 2.5%, 5%, and 10% (v:v)] for 30 days. The activities of superoxide dismutase and catalase enzymes decreased in responses to the gas oil presence by an average of 83% and 49%, respectively. In contrast, the activities of the ascorbate peroxidase and glutathione peroxidase enzymes displayed an upward trend in the groups cultured in oil-contaminated media. The gas oil contaminant increased total phenol and flavonoid accumulation, reflecting the variation in secondary metabolism. According to the 1,2-diphenyl-2-picrylhydrazyl radical scavenging, the 2.5% gas oil treatment resulted in the highest antioxidant activity (48 μg mL⁻¹). The highest scavenging activity of nitric oxide radicals (IC₅₀ = 272 μg mL⁻¹) was observed in the treatment with the highest gas oil concentration (10%). Also, this treatment showed an excellent ability to chelate Fe⁺² ions (IC₅₀ = 205 μg mL⁻¹). The IC₅₀ values of methanolic extract for nitric oxide scavenging activity and metal chelating ability were significantly reduced by increasing gas oil concentration in the treatments. With increasing the gas oil concentration, malondialdehyde content as a criterion measure of lipid peroxidation level showed significant reduction. These results show that P. florida is resistant to and a compatible mushroom with oil pollutants. Also, the activity of glutathione peroxidase and the ascorbate-glutathione cycle detoxify nitric oxide radicals and products of reactive oxygen species–induced lipid peroxidation in the gas oil treatments.

Graphical abstract

KEYWORDS:

• Antioxidant
• enzyme
• gas oil
• malondialdehyde
• nitric oxide
• Pleurotus florida

ACKNOWLEDGMENTS

The authors would like to acknowledge Dr. Mohadeseh Laripoor and Fatemeh Katuzian, Department of Microbiology, Faculty of Science, North Branch, Islamic Azad University, Tehran, Iran, for technical suggestions.

DISCLOSURE STATEMENT

No potential conflict of interest was reported by the author(s).