Compact organ-tissue electrophoresis system (CORES)

Abstract

Electrophoretic tissue clearing has been a commonly used laboratory method since the early twentieth century. Infrastructure for standard procedures has yet to be formed. In particular, control of the heat produced by electrophoresis, the voltage applied to the electrodes, the resistance, and the speed of liquid circulation create difficulty for researchers. We aimed to develop a compact organ electrophoresis system that enables the researcher to have easy, rapid, and inexpensive working opportunities. The system includes an electronic control unit, a liquid tank, a temperature control unit, and an electrophoresis chamber. The control unit software can keep the system stable by using information on temperature and circulation rate received through the sensors using the feedback principle. Corrosion and particle collection are reduced to a minimum as platinum wires are used for electrophoresis electrodes. A temperature control unit can heat and cool via a liquid tank base. The CORES is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. It assures efficient, rapid, and consistent tissue clearing. The system was stable with 72 h of continuous operation. Patent applications and trial version studies for introducing the system to researchers are still in progress.

Keywords:

• Tissue clearing
• CLARITY
• electrophoresis
• imaging

Authors’ Contributions

Conceptualisation: [Aysegul Gungor Aydin, Erdinc Sahin Conkur, Esat Adiguzel]; Methodology: [Aysegul Gungor Aydin, Esat Adiguzel]; Writing - original draft preparation: [Aysegul Gungor Aydin]; Writing - review and editing: [Aysegul Gungor Aydin, Erdinc Sahin Conkur, Esat Adiguzel] Visualisation: [Aysegul Gungor Aydin]; Funding acquisition: [Esat Adiguzel]; TUBITAK 1005-114S407.

Disclosure statement

The authors report there are no competing interests to declare.

Additional information

Funding

This work was funded by the Scientific and Technological Research Council of Turkey and (the National New Ideas and New Products Research Funding Program; 1005-114S407) to Esat Adiguzel.