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Abstract
Objective
The present research deals with sequential optimization strategy based on Central Composite Design to optimize the process variables for efficient production of Clitoria teratea (CLT) synthesized silver nanoparticles (AgNPs) using biological synthesis.
Methods
Two substantial factors influencing the dependent variables viz UV-visible absorbance, particle size, zeta potential and polydispersity index (PDI) were identified as NaOH concentration, RH concentration, temperature as independent variables. In-vitro and ex-vivo studies of prepared CLT-AgNPs gel and marketed gel were carried out using dialysis membrane and egg membrane, respectively. In addition, antimicrobial study was also performed on the bacterial strains.
Results
The particles size (114 nm), PDI (0.45), and zeta potential (−29.5 mV) of optimized formulation were found, respectively. In-vitro profile of AgNPs from prepared CLT-AgNPs gel was noted (95.6%) in 8 h. It was found that the prepared CLT-AgNPs gel stimulates fibroblast and agranulocytosis development resulting better and timely wound healing.
Conclusions
The prepared CLT-AgNPs gel can be as a potential substitute in the management and treatment of acute and chronic wounds.
GRAPHICAL ABSTRACT
Acknowledgments
The authors want to thank DPSRU University in New Delhi, India for their invaluable assistance and encouragement with this manuscript.
Author contributions
The manuscript was written by RJ and RS created the tables and figures. HP oversaw the work while TG and RB polished the language. All authors have agreed to the manuscript’s final version.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data Availability statement
The datasets generated and analyzed during the current research work are available upon request from the first author.