EFFECT OF GROWTH MEDIUM ON POTENTIAL OF Streptomyces Anulatus SPORES TO INDUCE INFLAMMATORY RESPONSES AND CYTOTOXICITY IN RAW264.7 MACROPHAGES

Abstract

Epidemiological studies have shown an association between microbial growth in buildings and increased risk of respiratory symptoms and disease related to inflammatory reactions in the inhabitants. The current study examined the affects of growth conditions of Streptomyces anulatus, isolated from indoor air of a moldy building, on the inflammatory potential of spores of this microbe. Spores were harvested from 15 growth media formulations, applied to RAW264.7 macrophages (10⁵, 10⁶, or 10⁷