Induction of Cytotoxicity and Production of Inflammatory Mediators in RAW264.7 Macrophages by Spores Grown on Six Different Plasterboards

Abstract

Dampness and microbial growth in buildings are associated with respiratory symptoms in the occupants, but details of the phenomenon are not sufficiently understood. The current study examined the effects of growth conditions provided by six plasterboards on cytotoxicity and inflammatory potential of the spores of Streptomyces californicus, Penicillium spinulosum, Aspergillus versicolor, and Stachybotrys chartarum. The microbes were isolated from mold problem buildings and thereafter grown on six different plasterboards. The spores were harvested, applied to RAW264.7 macrophages (10⁴, 10⁵, 10⁶ spores/10⁶