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Abstract
Background: Paraquat (PQ) plays an important role in agricultural production due to its highly effective herbicidal effect. However, it has led to multiple organ failure in those who have been poisoned, with damage most notable in the lungs and ultimately leading to death. Because of little research has been performed at the genetic level, and therefore, the specific genetic changes caused by PQ exposure are unclear.
Methods: Paraquat poisoning model was constructed in Sprague Dawley (SD) rats, and SD rats were randomly divided into Control group, paraquat (PQ) poisoning group and Anthrahydroquinone-2,6-disulfonate (AH2QDS) treatment group. Then, the data was screened and quality controlled, compared with reference genes, optimized gene structure, enriched at the gene expression level, and finally, signal pathways with significantly different gene enrichment were screened.
Results: This review reports on lung tissues from paraquat-intoxicated Sprague Dawley (SD) rats that were subjected to RNA-seq, the differentially expressed genes were mainly enriched in PI3K-AKT, cGMP-PKG, MAPK, Focal adhesion and other signaling pathways.
Conclusion: The signaling pathways enriched with these differentially expressed genes are summarized, and the important mechanisms mediated through these pathways in acute lung injury during paraquat poisoning are outlined to identify important targets for AH2QDS treatment of acute lung injury due to paraquat exposure, information that will be used to support a subsequent in-depth study on the mechanism of PQ action.
Author contributions
N L, Y H and Y Y were responsible for conception and designing the study, S Q X, H F W and X X W was responsible in data gathering, J Q, Q L,J C P,L H L and M L were responsible for data analysis, N L,J Q drafted the manuscript, J J Y and X R L responsible for the critical review of the paper. All authors participated in interpretation of the data.
Disclosure statement
The authors declare that they have no competing interests.
Availability of data and materials
The Illumina paired-ended sequenced Raw reads were filtered (fltered) using the fastp to remove low quality reads (https://github.com/OpenGene/fastp). The filtered (fltered) data is compared to the reference sequence. Reference genome and gene model annotation files (fles) were downloaded from genome website directly. (https://www.ncbi.nlm.nih.gov/assembly/GCF_000001895.5#/def).
Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG), we used the R package cluster Profiler51 (Profler51) to perform KEGG functional enrichment analysis of diferentially expressed genes.
Animal ethics
This study has been approved by the Ethics Committee of the First Affiliated Hospital of Hainan Medical University, and was carried out in accordance with the ethical standards of experimental animals. [2020 (Research) No. (97)] on July 8, 2020.