Abstract
Aims
The aim of this study was to investigate the impact of three single nucleotide polymorphisms (SNPs) within X-Ray Repair Cross Complementary Group 2 (XRCC2) gene and additional gene- abdominal obesity (AO) interaction with endometrial carcinoma (EC) risk.
Methods
Hardy–Weinberg equilibrium was tested for all participants by using SNPstats (online software: http://bioinfo.iconcologia.net/SNPstats). The best SNP–SNP and gene–AO interaction combination among three SNPs within XRCC2 gene and AO was screened using generalized multifactor dimensionality reduction (GMDR).
Results
We employed the logistic regression analysis showed that rs718282-T allele is associated with increased EC risk, adjusted ORs (95%CI) were 1.67 (1.23–2.04). However, we did not find statistical association between rs3218536, and rs3218384 and EC susceptibility. GMDR analysis was used for SNP-SNP- and gene-abdominal obesity analysis. The cross-validation consistency and the testing accuracy for the interaction were calculated. The two-locus model between rs718282 and AO had a testing accuracy of 60.11%, which was significant at the p < .001 level, and this two- locus model was considered as the best model. It provided statistical evidence for rs718282 gene–AO interaction effects. The results indicated that AO influenced the EC risk depending on the rs718282 genotypes. Compared with non- AO subjects with rs718282–CC genotype, AO subjects with rs718282-CT or TT genotype had the highest EC risk, OR (95%CI) was 2.83 (1.67 − 4.02), after covariates adjustment.
Conclusions
Both the rs718282- T allele, and its interaction with AO were associated with increased EC risk.
Acknowledgements
We appreciate the cooperation of the families and individuals who cooperated in this study.
Authors’ contributions
All authors have read and approved the manuscript.
Wenjuan Tian: definition of intellectual content, literature research, clinical studies
Siyu Cao: experimental studies, data acquisition, data analysis.
Wei Zhang: experimental studies and manuscript preparation
Chenlian Quan: statistical analysis.
Meiqin Zhang: guarantor of integrity of the entire study, study concepts and manuscript editing.
Yan Huang: guarantor of integrity of the entire study, study concepts, study design and manuscript review.
Ethics approval and consent to participate
Each participant understood the process of the study and signed a written informed consent before the start of the study. All study protocols of the current study were approved by ethics committee of Shanghai Medical College of Fudan University. All methods were performed in accordance with the Declaration of Helsinki.
Consent for publication
Not applicable.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Availability of data and material
The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.