Lactobionic acid production via mutant cellobiose dehydrogenase/laccase continuous enzymatic regeneration of electron acceptors

Abstract

Lactobionic acid (LBA) has diverse number of applications in pharmaceutical, food, chemical and nanotechnology fields. Bioproduction of LBA by bi-enzymatic cellobiose dehydrogenase (CDH)/laccase (LAC) is a system where CDH catalyses oxidation of lactose to LBA, whilst LAC has, at the same time, ability to regenerate the redox mediator as electron acceptor for CDH. In this study, CDH mutants derived from Phanerochaete chrysosporium produced in Pichia pastoris were used to determine LBA production rate with varying number of redox mediators. The highest specific productivity was observed for triple mutant CDH (tmCDH) for all redox mediators used in this study. The notable redox mediator was hydroquinone where specific productivity was 29.14 g L⁻¹ kU⁻¹ for tmCDH while specific productivity with DCIP was 27.2 g L⁻¹ kU⁻¹, with ABTS showed 19.1 g L⁻¹ kU⁻¹.

Graphic Abstract

Key Points

• Lactobionic acid production using bi-enzymatic system, CDH/LAC.

• Regeneration of different redox mediators.

• Recombinant mutants used in reaction system.

Keywords:

• Trametes versicolor
• Phanerochaete chrysosporium
• hydroquinone
• guaiacol

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Author contributions

RGE and MB managed experiments and wrote draft version of the article. MB, AMB and RP contributed new analytical tools and methods and analysed data. RP conceived and designed research. MB and AMB written draft version of the article. MB analysed data and wrote final version of the article.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

All data generated or analysed during this study are included in this published article and its supplementary information files.

Additional information

Funding

This work was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia: FC - Grant No. 451-03-47/2023-01/200168; ICTM - Grant No. 451-03-47/2023-01/200026.