Astaxanthin prevents bone loss in osteoporotic rats with palmitic acid through suppressing oxidative stress

ABSTRACT

Objectives

The study aimed to assess the role of Astaxanthin (ATX) in palmitic acid(PA) -induced bone loss in Ovariectomized(OVX) rats.

Methods

In the OVX rat model, we observed that PA affects bone metabolism and accelerates bone loss. Additionally, treatment with ATX was able to suppress the deleterious effects of PA and a simultaneous decrease in serum MDA levels and an increase in SOD was observed.

Results

In addition, rats treated with ATX were observed to have significantly increased bone mass and elevated activity of SIRT1 and SOD2 in bone tissue. When MC3T3-E1 and RAW264.7 cells induced osteoblast and osteoclast differentiation, the ATX intervention was able to significantly restore the restriction of osteogenic differentiation and the up-regulation of osteoclast differentiation with PA therapy. Furthermore, we confirm that PA damage to cells is caused by increased oxidative stress, and that ATX can target and modulate the activity of SIRT1 to regulate the levels of oxidative stress in cells.

Conclusion

Summarizing, ATX may inhibit PA-induced bone loss through its antioxidant properties via the SIRT1 signaling pathway.

GRAPHICAL ABSTRACT

KEYWORDS:

• Astaxanthin
• Osteoporosis
• Palmitic acid
• Oxidative stress
• Bone metabolism

Acknowledgements

This study was supported by a grant from Health Research Project of Anhui Province (AHWJ2023A20550), Wuhu Science and Technology Project (2022jc61), Outstanding Youth Project of Anhui University Natural Science (2023AH020050) and Anhui University Natural Science Key Project (KJ2021A0822).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are available from the corresponding author [ZS Tao], upon reasonable request.

Additional information

Funding

This work was supported by Health Research Project of Anhui Province [Grant Number AHWJ2023A20550].