A hybrid composite of Polypyrole/carboxymethyl cellulose/MWCNT fiber with antimicrobial properties and Sb3+ determination on a glassy carbon electrode

ABSTRACT

In order to prepare fiber-type nanohybrid composites for electrochemical and antimicrobial applications, polypyrrole was combined with carboxymethylcellulose and multiwalled carbon nanotubes (PPy/CMC/MWCNTS). These composite materials were synthesized using ultrasonication and in situ polymerization. By using analytical methods, the structure and morphology of the synthesized nanohybrid composite material were confirmed. Slurry of fiber-type PPY/CMC/MWCNT composites synthesized in ethanol was deposited as a thin-uniform layer with conductive nafion (5% ethanolic solution of nafion) binder. Based on electrochemical measurements in a phosphate buffer medium, Sb³⁺ ions have linear responses between 0.1 nM and 0.01 mM, which is known as linear dynamic range (LDR). A sensor’s sensitivity (22.4304 µAµM⁻¹cm⁻²) is calculated using the LDR slope by considering the GCE surface area (0.0316 cm²). A signal-to-noise ratio of 3 is used to estimate the lower limit of detection which is equal to 96.82 ± 4.84 pM. Moreover, the antibacterial activity of the obtained polypyrrole/carboxymethylcellulose/MWCNT composite has also been evaluated “against Gram-positive bacteria B. subtilis along with S. aureus, as well as Gram-negative bacteria, P. aeruginosa along with Escherichia coli by utilizing” autoclave of agar media. In electrochemical analysis, the proposed Sb³⁺ cationic sensor exhibits appreciable reproducibility, response time, stability, and outstanding outcome in analysis of real samples. In the field of metal ion sensor, this reliable method might be prospective in the recent future.

摘要

为了制备用于电化学和抗菌应用的纤维型纳米杂化复合材料, 将聚吡咯与羧甲基纤维素和多壁碳纳米管（PPy/CMC/MWCNTS）相结合. 这些复合材料是通过超声处理和原位聚合合成的. 通过分析方法, 证实了合成的纳米杂化复合材料的结构和形态. 将在乙醇中合成的纤维型PPY/CMC/MWCNT复合材料浆料与导电nafion（5%的nafion乙醇溶液）粘合剂沉积为均匀的薄层. 根据在磷酸盐缓冲介质中的电化学测量,Sb3+离子具有0.1 nM和0.01 mM之间的线性响应, 这被称为线性动态范围（LDR）. 通过考虑GCE表面积（0.0316 cm2）, 使用LDR斜率计算传感器的灵敏度（22.4304 µAµM-1 cm-2）. 使用3的信噪比来估计检测的下限,该下限相等96.82 ± 4.84 pM. 此外,还评估了所获得的聚吡咯/羧甲基纤维素/MWCNT复合物的抗菌活性,“通过使用”琼脂培养基的高压釜“对抗革兰氏阳性菌枯草芽孢杆菌和金黄色葡萄球菌, 以及革兰氏阴性菌、铜绿假单胞菌和大肠杆菌.” 在电化学分析中,所提出的Sb3+阳离子传感器在实际样品的分析中表现出可观的再现性、响应时间、稳定性和出色的结果. 在金属离子传感器领域,这种可靠的方法在最近的将来可能是有前景的.

KEYWORDS:

• Composites PPY/CMC/MWCNTS Fiber
• Sb³⁺ ion sensor
• electrochemical method
• glassy carbon electrode
• antimicrobial study
• environmental safety

关键词:

• 复合材料PPY/CMC/MWCNTS纤维
• Sb3+离子传感器
• 电化学方法
• 玻璃碳电极
• 抗菌研究
• 环境安全

Acknowledgements

This research work was funded by the Institutional Fund Project under grant no. (IFPHI-279-130-2020). Therefore, authors gratefully acknowledge technical and financial support from the Ministry of Education and King Abdulaziz University, DSR, Jeddah, Saudi Arabia.

Disclosure statement

No potential conflict of interest was reported by the authors.

Highlights

• A fiber-type nanohybrid PPY/CMC/MWCNTS composite was prepared for electrochemical and antimicrobial applications.

• To investigate the Sb3+ cationic sensor using the I-V method on a glassy carbon electrode coated with PPY/CMC/MWCNTS composite.

• Antibacterial study results showed that PPY/CMC/MWCNTS composite was most effective against Bacillus bacteria, as well as Staphylococcus aureus, respectively.

• A lower limit of detection is equal to 96.82 ± 4.84 pM at the signal-to-noise ratio at an SNR of 3 (signal-to-noise ratio).

Additional information

Funding

The work was supported by the King Abdulaziz University [IFPHI-279-130-2020].