Bio-Fabrication and Efficiency Analysis of Annona Muricata Extract Coated on Bamboo Fiber for Wound Healing Application

ABSTRACT

This study focusses on the bio-fabrication of leaf extracts of Annona muricata dyed bamboo woven fabric and its application in wound healing. Annona muricata was exposed to ultrasonic-assisted extraction (UAE) with acetone for fabric finishing. Bioactivity is characterized and employed in textile fabric coating to evaluate the efficacy of its antibacterial and wound healing characteristics. The treated fabric was then tested for antibacterial activity using gram-positive and gram-negative bacteria, aroma, color intensity, olfaction, and invivo wound healing. The invivo test was carried out in wistar rats for 21 days for 50%, 75%, and 100%. According to the results of the analysis, the extract is a good antibacterial agent against gram-positive bacteria (Bacillus subtilus) and gram-negative bacteria (Pseudomonas sp.), which aids in the prevention of bacteria and other germs from sticking to the injured area since the leaf extracts have high antibacterial properties. The wound healing impact of the plant extract coated with bamboo fabric on in vivo wound models in rats (Wistar albino rats) demonstrated a significant increase in wound closure, showing the efficacy of the coated fabric.

摘要

研究了番荔枝叶提取物对竹编织物的染色及其在伤口愈合中的应用. 用丙酮对番荔枝进行超声波辅助提取（UAE)，用于织物整理. 生物活性物质被表征并用于纺织品涂层，以评估其抗菌和伤口愈合特性的功效.然后使用革兰氏阳性菌和革兰氏阴性菌测试处理过的织物的抗菌活性、香气、颜色强度、嗅觉和内脏伤口愈合. 在 wistar 大鼠中进行了为期21天的invivo试验. 分别为50%、75%和100%. 根据分析结果，该提取物是对革兰氏阳性菌（枯草芽孢杆菌）和革兰氏阴性菌（假单胞菌属）的良好抗菌剂，由于叶提取物具有高抗菌性能，因此有助于防止细菌和其他细菌粘附在损伤区域.用竹织物包裹的植物提取物对大鼠（Wistar白化大鼠）体内伤口模型的伤口愈合影响表明，伤口闭合显著增加，显示了包裹织物的功效.

KEYWORDS:

• Annona muricata
• bamboo fabric
• GCMS
• FTIR
• Olfaction assay
• antimicrobial activity
• antioxidant activity
• wound dressing
• wound healing

关键词:

• 番荔枝
• 竹织物
• 嗅觉测定
• 抗菌活性
• 抗氧化活性
• 伤口敷料
• 伤口愈合

Acknowledgements

This work is part of final year B.Tech Biotechnology project of first and second authors. The authors gratefully acknowledge the Department of Biotechnology and Sri Shakthi Institute of Engineering and Technology for providing an ambient environment for the successful completion of the project.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Highlights          

• The leaf extracts of Acalypha indica and Tectona grandis were made into consortia with different concentrations (50% 75%, and 100%) and coated on bamboo woven fiber material for wound dressing applications.

• The bamboo woven fabric was utilized as a textile substrate for this research. Chitosan is used as a cross-linking agent and potassium alum was used as a mordant, and engrossed with various concentrations of the bioactive extract.

• This treated fabric was further evaluated for antimicrobial assay using gram-positive and gram-negative bacteria, fragrance test, color intensity, and olfaction test, followed by in vivo wound healing assay.

• It is asserted that natural extracts coated on bamboo-based dressing materials offer beneficial properties for wound healing and allows the attire and textile industry to utilize bio-extract-treated fabric in developing innovative and hygienic attire products in the future.