https://orcid.org/0000-0001-7594-2615
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ABSTRACT
Cannabis sativa L. is an extremely variable species. Even within the same cultivar plants can significantly differ in the content and profile of cannabinoids. Therefore, the best method for production of uniform plants and standardized raw material is vegetative propagation using clones. The aim of this study was to determine the content of cannabidiolic acid (CBDA), cannabidiol (CBD), Δ9-tetrahydrocannabinolic acid (Δ9-THCA), Δ9-tetrahydrocannabinol (Δ9-THC), cannabichromene (CBC), cannabigerol (CBG), and cannabinol (CBN) in clonally propagated plants of industrial hemp. One hundred and thirty-nine plants representing 17 different hemp genotypes were regenerated in vitro, hardened, and grown in a vegetation hall until harvest. Single plants of each accession were analyzed using high-performance liquid chromatography with UV/diode-array detection (HPLC-DAD/UV). The results revealed significant variability in the total cannabinoid content (0.55–5.18% in dry weight) among tested genotypes and within the Epsilon 68 cultivar. The highest content of total CBD (4.410%) was recorded for EPS/40 genotype, while the level of total Δ9-THC was below the allowed threshold (0.3%). Therefore, we can conclude that some clonally propagated plants provided reproducible hemp material as a potential source of cannabidiol. The results of this study will be useful for breeding and early selection of hemp genotypes.
摘要
大麻是一个极易变异的物种. 即使在同一栽培品种中，大麻素的含量和分布也可能存在显著差异. 因此，生产统一植物和标准化原料的最佳方法是使用无性繁殖. 本研究旨在测定工业大麻克隆繁殖植物中大麻二酚酸（CBDA）、大麻二酚（CBD）、Δ9-四氢大麻酚酸（Δ9-THCA）、Δ9-THC、大麻色素烯（CBC）、大麻酚（CBG）和大麻酚（CBN）的含量. 代表17种不同大麻基因型的139株植物在体外再生、硬化并在植被大厅中生长，直到收获. 使用具有UV/二极管阵列检测的高效液相色谱法（HPLC-DAD/UV）分析每个登录的单株. 结果显示，在受试基因型之间和受试基因组内，大麻素总含量（干重为0.55–5.18%）存在显著差异. 结果显示，在测试的基因型和Epsilon 68品种中，大麻素总含量存在显著差异（干重为0.55–5.18%）. EPS/40基因型的总CBD含量最高（4.410%），而总Δ9-THC水平低于允许的阈值（0.3%）. 因此，我们可以得出结论，一些克隆繁殖的植物提供了一种可再生的大麻材料，作为大麻二酚的潜在来源。研究结果对大麻基因型的选育和早期选择具有一定的指导意义.
Acknowledgements
The authors would like to express their gratitude to Professor Henryk Burczyk for providing seeds of the Henola cultivar and to Dr Karolina Wielgus for her assistance and support.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Ethical approval
We confirm that all the research meets ethical guidelines and adheres to the legal requirements of the study country. The research does not involve any human or animal welfare-related issues.