Genome-Wide Analysis of the PLATZ Gene Family and Identification of Seed Development-Related Genes in Flax [Linum usitatissimum L.]

ABSTRACT

The plant AT-rich sequence and zinc-binding proteins (PLATZ) are a new class of zinc-dependent DNA-binding proteins that play important roles in plant growth and development. But it has not been systematically identified in flax (Linum usitatissimum L.), as an important seed crop with high nutritional value. In this study, 28 LuPLATZ proteins were identified from flax and divided into six phylogenetic groups. Fragment duplication was an important driving factor in the evolution of LuPLATZ genes. The RNA-seq results showed that the LuPLATZ genes had different expression patterns. Quantitative analysis of 16 LuPLATZ genes was performed, and the qRT-PCR results were consistent with the RNA-seq results. The expression of LuPLATZ6, LuPLATZ23, and LuPLATZ27 increased significantly during seed development. The observed change in the expression pattern suggests that these three genes could have significant regulatory functions during seed formation. These three LuPLATZ proteins were localized to the nucleus, suggesting that they could be conventional transcription factors. LuPLATZ23 and LuPLATZ27 were co-expressed with LuNAC1 and LuNAC75, suggesting an interactive relationship. This study aims to offer a theoretical foundation for high-yielding molecular breeding of flax.

摘要

植物富含 AT 序列和锌结合蛋白 (PLATZ) 是一类新型锌依赖性 DNA 结合蛋白，在植物生长和发育中发挥重要作用。但亚麻(Linum usitatissimum L.)作为一种具有高营养价值的重要种子作物尚未得到系统鉴定。在这项研究中，从亚麻中鉴定出 28 个 LuPLATZ 蛋白，并将其分为 6 个系统发育组。片段重复是LuPLATZ基因进化的重要驱动因素。 RNA-seq结果显示LuPLATZ基因具有不同的表达模式。对16个LuPLATZ基因进行定量分析，qRT-PCR结果与RNA-seq结果一致。 LuPLATZ6、LuPLATZ23和LuPLATZ27的表达在种子发育过程中显着增加。观察到的表达模式的变化表明这三个基因在种子形成过程中可能具有重要的调节功能。这三种 LuPLATZ 蛋白定位于细胞核，表明它们可能是常规转录因子。 LuPLATZ23 和 LuPLATZ27 与 LuNAC1 和 LuNAC75 共表达，表明存在交互关系。本研究旨在为亚麻高产分子育种提供理论基础。

KEYWORDS:

• Flax
• PLATZ
• gene family
• seed development
• candidate genes

关键词:

• 亚麻
• 普拉茨
• 基因家族
• 种子发育
• 候选基因

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/15440478.2024.2321534