Identification and expression analysis of YABBY family genes in Platycodon grandiflorus

ABSTRACT

Platycodon grandiflorus set ornamental, edible, and medicinal plant with broad prospects for further application development. However, there are no reports on the YABBY transcription factor in P. grandiflorus. Identification and analysis of the YABBY gene family of P. grandiflorus using bioinformatics means. Six YABBY genes were identified and divided into five subgroups. Transcriptome data and qRT-PCR were used to analyze the expression patterns of YABBY. YABBY genes exhibited organ-specific patterns in expression in P grandiflorus. Upon salt stress and drought induction, P. grandiflorus presented different morphological and physiological changes with some dynamic changes. Under salt treatment, the YABBY gene family was down-regulated; PgYABBY5 was up-regulated in leaves at 24 h. In drought treatment, PgYABBY1, PgYABBY2, and PgYABBY3 were down-regulated to varying degrees, but PgYABBY3 was significantly up-regulated in the roots. PgYABBY5 was up-regulated gradually after being down-regulated. PgYABBY5 was significantly up-regulated in stem and leaf at 48 h. PgYABBY6 was down-regulated at first and then significantly up-regulated. The dynamic changes of salt stress and drought stress can be regarded as the responses of plants to resist damage. During the whole process of salt and drought stress treatment, the protein content of each tissue part of P grandiflorus changed continuously. At the same time, we found that the promoter region of the PgYABBY gene contains stress-resistant elements, and the regulatory role of YABBY transcription factor in the anti-stress mechanism of P grandiflorus remains to be studied. PgYABBY1, PgYABBY2, and PgYABBY5 may be involved in the regulation of saponins in P. grandiflorus. PgYABBY5 may be involved in the drought resistance mechanism in P. grandiflorus stems and leaves. This study may provide a theoretical basis for studying the regulation of terpenoids by the YABBY transcription factor and its resistance to abiotic stress.

KEYWORDS:

• YABBY transcription factor
• Platycodon grandiflorus
• abiotic stress
• qRT-PCR
• expression patterns

Acknowledgments

The authors thank the teachers and students of the research group for their technical language assistance. They also thank the editors and reviewers for their reading and comments on the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

All data generated or analyzed during this study are included in this published article and its supplementary information files https://doi.org/10.1080/15592324.2022.2163069.

Author contributions

LK, JS and ZJ conceived the research. LK, JS, ZJ, and WR designed the experiment, contributed to data analysis, and wrote the paper. ZW and MZ contributed to qRT-PCR data analysis and discussion. LW, XL, WM, and JX revised the paper. All authors contributed to the article and approved the submitted version.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/15592324.2022.2163069

Additional information

Funding

National Key Research and development Project, research and demonstration of collection, screening and breeding technology of ginseng and other genuine medicinal materials, Project No. 2021YFD1600901; Talent training project supported by the central government for the reform and development of local colleges and Universities(ZYRCB2021008); Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education and Heilongjiang Touyan Innovation Team Program (Grant Number: [2019] No. 5); Graduate Innovation Project of Heilongjiang University of Chinese Medicine (2022yjscx010).