Gasotransmitter ammonia accelerates seed germination, seedling growth, and thermotolerance acquirement in maize

ABSTRACT

Ammonia (NH₃), as an intermediate product of nitrogen metabolism, is recognized as a novel gasotransmitter (namely gaseous signaling molecule), its signaling role being revealed in plants. NH₃ exists in two different chemical forms, namely the weak base (free molecule: NH₃) and the weak acid (ammonium: NH₄⁺), which are generally in equilibrium with each other in plants. However, the effect of NH₃ on seed germination, seedling growth, and thermotolerance acquirement in maize remains unclear. Here, maize seeds were imbibed in the different concentrations of NH₃·H₂O (NH₃ donor), and then germinated and calculated seed germination rate at the various time points. Also, the 60-h-old seedlings were irrigated in the different concentrations of NH₃·H₂O, and then subjected to heat stress and counted survival rate. The data implied that the appropriate concentrations (6, 9, and 12 mM) of NH₃·H₂O accelerated seed germination as well as increased seedling height and root length compared with the control without NH₃ treatment. Also, the suitable concentrations (2 and 4 mM) of NH₃·H₂O improved tissue vitality, relieved an increase in malondialdehyde content, and enhanced survival rate of maize seedlings under heat stress compared with the control. These results firstly suggest that NH₃ could accelerate seed germination, seedling growth, and thermotolerance acquirement in maize.

KEYWORDS:

• Ammonia
• seed germination
• seedling growth
• seedling thermotolerance
• maize

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/15592324.2022.2163338

Additional information

Funding

This work is supported by National Natural Science Foundation of China (32160065).