Salicylic Acid Enhances Growth, Photosynthetic Performance and Antioxidant Defense Activity Under Salt Stress in Two Mungbean [Vigna radiata (L.) R. Wilczek] Variety

ABSTRACT

Salt is regarded as a main cause for reduced yield under challenging conditions. Mungbean, a valuable protein crop, is sensitive to salt stress, leading to yield shortage. The growth hormone, salicylic acid (SA), enhances several processes necessary to confer salt tolerance and relieves poor agricultural yield. Seeds of mungbean were initially pretreated with SA (0.5 mM) for 4 h before sowing, while under a cumulative combination of SA + salt regimes: control, SA, 100 mM, SA +100 mM, 200 mM and SA +200 mM. Our study examined photosynthesis parameters such as photosynthetic pigment concentration, chlorophyll a fluorescence, protein, proline, and antioxidant enzymes in plants subjected to single and combined SA + salt stress concentrations. The result showed a greater decline in SPAD and photosynthetic quantum yield under 200 mM NaCl at 43% in Var. 145 than in Var. 155 at 32% compared to 11% in SA +100 mM and 34% in SA + 200 mM treatments in both varieties. Var. 145 was found to be more sensitive to 100 and 200 mM NaCl salt stress. In Var. 155, chlorophyll a and chlorophyll b concentrations were higher under control 52%, SA + 100 mM 49%, and SA +200 mM 42% than in Var. 145 at 51%, 38%, and 31%. Protein and proline revealed a higher content in Var. 155 in contrast to the lower activity in Var. 145. The enhanced performance of the Var. 155 exposed to SA + salt stress was followed by an increase in the activities of peroxidase (POD), CAT while the activity of MDA revealed a significant increase in Var. 145 under 100 mM 43% and 200 mM 48% NaCl treatment compared to Var. 155, which had 38% and 34%. The above results suggest that SA-treated Var. 155 confers tolerance to salt stress and is accompanied with a high osmoprotectant responses as provided by SA in Var. 155 than Var. 145. The potency of SA in providing salt tolerance capacity to plants is a future research interest to maintain sustainable yield in mungbean seedlings.

KEYWORDS:

• Salinity
• salicylic acid
• mungbean
• tolerance
• enzymes
• yield

Acknowledgments

We give our sincere thanks to all authors who provided support and special assistance to Coimbra Group scholarship for providing a research fellowship to Esther for the completion of this study.

Disclosure statement

No potential conflict of interest was reported by the authors.

Author contributions

EO: methodology and writing. CU: conceptualization, methodology, and investigation. ES: methodology, conceptualization, and formal analysis. EI: methodology and writing. VO and AO: formal analysis, statistical analysis, and editing.

Additional information

Funding

The work was supported by the Coimbra Group Scholarship Coimbra Group Scholarship.