ABSTRACT
Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.
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Disclosure statement
No potential conflict of interest was reported by the author(s).
Authors’ contributions
Ke Li and Pengfei Wang designed the study. Ke Li, Qingtian Zhang, Huiping Liu, Fengxia Wang, Ao Li, Tingting Ding, Qian Mu and Hongjun Zhao performed the research and analyzed the data. Ke Li and Pengfei Wang contributed to writing the article. All authors reviewed and approved the manuscript.