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ABSTRACT
Plant metabolism is constantly changing and requires input signals for efficient regulation. The mitochondrial calcium uniporter (MCU) couples organellar and cytoplasmic calcium oscillations leading to oxidative metabolism regulation in a vast array of species. In Arabidopsis thaliana, genetic deletion of AtMICU leads to altered mitochondrial calcium handling and ultrastructure. Here we aimed to further assess the consequences upon genetic deletion of AtMICU. Our results confirm that AtMICU safeguards intracellular calcium transport associated with carbohydrate, amino acid, and phytol metabolism modifications. The implications of such alterations are discussed.
Acknowledgments
The technical support of Karina Jiménez Durán at Unidad de Servicios de Apoyo a la Investigación y a la Industria (USAII, F.Q., UNAM) is acknowledged. The authors thank Esmeralda Mandujano-Nava and Israel González Vizueth for performing imaging experiments. Xóchitl Pérez-Martinez and César Cuevas-Velázquez provided useful conceptual feedback for the completion of this study. We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis TDNA insertion mutant strain.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Abbreviations
| MCU | = | mitochondrial calcium uniporter |
| MCUc | = | mitochondrial calcium uniporter holocomplex |
| MICU | = | mitochondrial calcium uniporter regulator |
| PAB | = | protoplast assay buffer |
| PCA | = | principal component analysis |
| PLS-DA | = | partial least squares-discriminate analysis |
| PES | = | protoplast enzyme solution |
| ROS | = | reactive oxygen species |
| RuR | = | ruthenium red |
| TCA | = | tricarboxylic acid cycle |
Author contributions
ER G-M, R R-R and C L-A performed investigation, conceptualization, and methodology.
JM G-A provided resources and supervision.
JC P-F provided project conceptualization, valuable methodology, software, data validation, formal analysis, investigation, data curation and aided during the writing process of the original draft.
M G-A Performed experimental conceptualization, supervision, project administration, funding acquisition, formal analysis, writing the original draft, manuscript review & editing.
Supplementary material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/15592324.2023.2271799.