Unveiling kiwifruit TCP genes: evolution, functions, and expression insights

ABSTRACT

The TEOSINTE-BRANCHED1/CYCLOIDEA/PROLEFERATING-CELL-FACTORS (TCP) gene family is a plant-specific transcriptional factor family involved in leaf morphogenesis and senescence, lateral branching, hormone crosstalk, and stress responses. To date, a systematic study on the identification and characterization of the TCP gene family in kiwifruit has not been reported. Additionally, the function of kiwifruit TCPs in regulating kiwifruit responses to the ethylene treatment and bacterial canker disease pathogen (Pseudomonas syringae pv. actinidiae, Psa) has not been investigated. Here, we identified 40 and 26 TCP genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes, respectively. The synteny analysis of AcTCPs illustrated that whole-genome duplication accounted for the expansion of the TCP family in Ac. Phylogenetic, conserved domain, and selection pressure analysis indicated that TCP family genes in Ac and Ae had undergone different evolutionary patterns after whole-genome duplication (WGD) events, causing differences in TCP gene number and distribution. Our results also suggested that protein structure and cis-element architecture in promoter regions of TCP genes have driven the function divergence of duplicated gene pairs. Three and four AcTCP genes significantly affected kiwifruit responses to the ethylene treatment and Psa invasion, respectively. Our results provided insight into general characters, evolutionary patterns, and functional diversity of kiwifruit TCPs.

KEYWORDS:

• Kiwifruit
• TCP gene family
• Actinidia chinensis
• Actinidia eriantha
• whole-genome duplication
• expression profiles
• conserved motif

Disclosure statement

No potential conflict of interest was reported by the author(s).

Author contributions

Zhicheng Wu designed the experiment and Donglin Li conducted the experiment. Haibo Li, Huimin Feng and Ping Qi contributed to transient expression assay and sample tissue collection. Donglin Li and Zhicheng Wu performed the data processing and drafted the manuscript. All authors have read and agreed the unpublished version of the manuscript.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/15592324.2024.2338985

Additional information

Funding

This study was supported by the Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province [210901084530323] [230306166273758] and National Natural Science Foundation of China [No. 32302588].