https://orcid.org/0000-0002-1776-2373
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ABSTRACT
Objective
The bruxism has a multifactorial etiology, in which molecular factors and genes play an important role. Dopaminergic pathways are pathways involved in functions such as motor control, reward, motivation, arousal and cognition. Genetic polymorphisms in the genes encoding Dopamine Receptor D2 (DRD2) and Ankyrin Repeat and Kinase Domain Containing 1 (ANKK1) have been reported in the literature associated with sleep disorders, migraine, personality, acute and chronic pain.
Methods
The present study aimed to assess whether genetic polymorphisms in DRD2 (rs6275 and rs6276) and ANKK1 (rs1800497) are associated with bruxism in construction men workers. A total of 113 male patients were evaluated. Bruxism was determined using a questionnaire, in which the patient self-reported the presence or absence of bruxism. For genetic analysis, saliva was collected from all individuals and the extraction of genomic DNA was performed to investigate the genetic polymorphisms rs6275 (C/T), rs6276 (A/G) and rs1800497 (C/T). The statistical significance was determined adopting an alpha = 0.05.
Results
Twenty-three (20.35%) patients reported at least one symptom of bruxism. Clenching was reported by 15.93% of the patients and grinding was reported by 14.16% of the patients. There was no statistical significance association between the studied genetic polymorphisms and bruxism (p > .05).
Conclusions
Our study did not observe an association between the genetic polymorphisms in DRD2 and ANKK1 and bruxism in a group of male construction workers.
KEYWORDS:
Abbreviations
| DRD2 | = | Dopamine Receptor D2 |
| ANKK1 | = | Ankyrin Repeat and Kinase Domain Containing 1 |
| DNA | = | Deoxyribonucleic Acid |
| DPs | = | Dopaminergic pathways |
| STREGA | = | STrengthening the REporting of Genetic Association Studies |
| PCR | = | Polymerase chain reaction |
Acknowledgments
The authors thank all participants of the study. This research was supported by FAPESP - The São Paulo Research Foundation (Erika Calvano Küchler 2015/06866-5 and 2016/08149-1).
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data Availability statement
The datasets generated and analyzed during this study are available from the corresponding author upon reasonable request.
Ethics Approval and Consent to Participate
All experiments were performed in accordance with applicable guidelines and regulations. The ethics committee of the number 2.802.708 CAAE 94262618.40000.0093, approved the samples collection and experiment. Informed written consent was obtained from all patients.
Additional information
Funding
Notes on contributors
Michelle Nascimento Meger
Michelle Nascimento Meger is the University professor and researcher at the Tuiuti University of Paraná (UTP), Brazil. She is a specialist in Oral and Maxillofacial Surgery and Traumatology, with a master’s degree and PhD. He is a full member of the Brazilian College of Oral and Maxillofacial Surgery and Traumatology.
Samantha Schaffer Pugsley Baratto
Samantha Schaffer Pugsley Baratto the University professor and researcher at the Unidombosco University Center (UniDomBosco), Brazil.
Bianca Cavalcante- Leao
Bianca Cavalcante-Leão is a professor in the postgraduate program in Dentistry at the Tuiuti University of Paraná and holds the position of head of the discipline Temporomandibular Disorder (TMD) and Orofacial Pain. Dr. Bianca has 20 years of clinical experience in this area, with her main research focus on bruxism.
Maria Eduarda Nunis Locks
Maria Eduarda Locks has a degree in dentistry from the University of the Region of Joinville (Univille) for 3 years, a master’s student in health and environment with completion in 2024. Conducts research in the area of endodontics and anatomy.
Camila Paiva Perin
Camila Paiva Perin is a Specialist, Master and Doctor in Endodontics, Specialist in Public Health, Adjunct Professor of the Dentistry course at the Tuiuti University of Paraná and professor of the Master’s in Dentistry program at the same university. She also works in the Brazilian public health service, the Unified Health System (SUS).
Gisele Maria Correr
Gisele Maria Correr is Adjunct Professor and Deputy Head of the Department of Restorative Dentistry at the Federal University of Paraná (UFPR). She has experience in the field of Dentistry, with an emphasis on Operative Dentistry and Dental Materials, working mainly on the following topics: bond strength (adhesion), tooth wear, biodegradation, material properties and dental erosion.
Natanael Henrique Mattos
Natanael H R Mattos is Professor and Coordinator of the Dentistry Course at the Tuiuti University of Paraná. PhD in Endodonticsfrom PUCPR and national and international speaker. Member of the Brazilian Society of Endodontics.
Christian Kirschneck
Christian Kirschneck is currently the head of the Department of Orthodontic at the University of Bonn in Germany. He received and completed his orthodontic specialist training at the Department of Orthodontics of the University of Regensburg. He graduated in both Dentistry and Molecular medicine also at the University of Regensburg. Dr. Kirschneck is member of numerous national and international scientific societies, such as the German Anatomical Society, the German and European Orthodontic and Dental Societies, the AAO and the World Federation of Orthodontists.
Erika Calvano Küchler
Erika Calvano Küchler is currently the head of the laboratory at the Department of Orthodontic (University of Bonn) and professor of the postgraduate program in Dentistry at Tuiuti University of Paraná. She received specialist training in Pediatric Dentist, later performed a master in Dentistry and PhD in Medical Sciences. She also has a Postdoc in Human Genetics.
Flares Baratto-Filho
Flares Baratto-Filho is the University professor and researcher at the University of Region of Joinville (Univille), Brazil. He is also coordinator of master’s degree in endodontics at Tuiuti University of Paraná (UTP), Brazil.