ABSTRACT
Background
Streptococcus mutans (S. mutans) is a pivotal cariogenic pathogen contributing to its multiple virulence factors, one of which is synthesizing exopolysaccharides (EPS). VicK, a sensor histidine kinase, plays a major role in regulating genes associated with EPS synthesis and adhesion. Here we first identified an antisense vicK RNA (ASvicK) bound with vicK into double-stranded RNA (dsRNA).
Objective
This study aims to investigate the effect and mechanism of ASvicK in the EPS metabolism and cariogenesis of S. mutans.
Methods
The phenotypes of biofilm were detected by scanning electron microscopy (SEM), gas chromatography-mass spectrometery (GC-MS) , gel permeation chromatography (GPC) , transcriptome analysis and Western blot. Co-immunoprecipitation (Co-ip) assay and enzyme activity experiment were adopted to investigate the mechanism of ASvicK regulation. Caries animal models were developed to study the relationship between ASvicK and cariogenicity of S. mutans.
Results
Overexpression of ASvicK can inhibit the growth of biofilm, reduce the production of EPS and alter genes and protein related to EPS metabolism. ASvicK can adsorb RNase III to regulate vicK and affect the cariogenicity of S. mutans.
Conclusions
ASvicK regulates vicK at the transcriptional and post-transcriptional levels, effectively inhibits EPS synthesis and biofilm formation and reduces its cariogenicity in vivo.
Disclosure statement
No potential conflict of interest was reported by the authors.
Author contributions
Y.T. Sun., H. Chen. and H.C. Mao. contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript; S.Y. Yang., M.M. Xu. And X. Qiao., contributed to data acquisition, analysis, and interpretation, critically revised the manuscript; L.W. He., contributed to data acquisition and analysis, critically revised the manuscript; D.Q. Yang, contributed to conception, design, data acquisition, analysis, and interpretation, critically revised the manuscript. All authors gave final approval and agree to be accountable for all aspects of the work.
Supplementary material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/20002297.2023.2204250