ABSTRACT
Objectives
The aims of this study were to select heat-killed lactic acid bacteria (HKL) with antibiotic activity and investigate the efficacy of this bacteria in maintaining periodontal parameters in healthy participants.
Materials and methods
An in vitro evaluation was conducted to assess the inhibitory efficacy of lactic acid bacteria against Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum. The effects of HKL administration on various parameters (plaque control record, bleeding on probing, and probing pocket depth) were assessed in a randomized, placebo-controlled trial. Participants in the test and placebo groups (n = 32) consumed oral tablets containing placebo or HKL daily for 8 weeks. Oral bacteria in supra-plaque and saliva were identified using 16S rRNA gene community profiling analysis.
Results
Heat-killed Ligilactobacillus salivarius CP3365 significantly (p < 0.05) decreased the viability of oral bacteria and was selected for clinical trials. Administration of HKL CP3365 significantly (p < 0.05) inhibited increases in each parameter. No changes in the relative abundance of P. gingivalis or F. nucleatum subsp. nucleatum were detected by HKL CP3365, but the relative abundance of oral bacteria (genera Porphyromonas, Fusobacterium, and Haemophilus) was significantly (p < 0.05) decreased.
Conclusion
HKL CP3365 effectively inhibited oral bacteria growth and was useful for maintaining periodontal health.
Clinical Trial Registration
[https://www.umin.ac.jp/ctr/index.htm], identifier [UMIN000045656].
Disclosure statement
S.S., Y. S., M.Y., and T.H. are employees of Asahi Quality and Innovations Ltd., which is affiliated with Asahi Group Holdings Ltd., and they prepared CP3365 and the placebo. T.S receives research consulting fees from Kyowa Trial Co., Ltd.
Author contributions
S. S. and T. S. conceived and designed the experiments. S. S. and Y. S. performed the experiments. S. S and M. Y. analyzed the data. S. S., T. S., and T. H. supervised the study. S. S., M. Y., T. S., and T. H. wrote the paper. All authors agreed to the final submitted and published version of the manuscript.
Data availability statement
The sequencing data presented in the study are deposited in https://www.ncbi.nlm.nih.gov/. DRR426262-DRR426509.
Supplementary Material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/20002297.2023.2250649