Parkinson’s disease alters the composition of subgingival microbiome

ABSTRACT

Aim

The current study aimed to test the hypothesis that Parkinson’s disease exacerbates periodontitis by altering its microbiome.

Materials and Methods

Clinical periodontal parameters were recorded. Subgingival samples from healthy controls, periodontitis patients (PD), and Parkinson’s patients with periodontitis (PA+PD) were analyzed using the checkerboard DNA-DNA hybridization technique for targeting 40 bacterial species typically associated with periodontal disease and health. Next-generation sequencing (NGS) of the 16S ribosomal RNA gene (V1-V3 regions) was performed to analyze the microbiome comprehensively.

Results

Parkinson’s patients had mild-to-moderate motor dysfunctions. Bleeding on probing was significantly increased in the PA+PD group compared to PD (p < 0.05). With checkerboard analysis, PA was associated with increased Treponema socranskii (p = 0.0062), Peptostreptococcaceae_[G-6] [Eubacterium]_nodatum (p = 0.0439), Parvimona micra (p < 0.0001), Prevotella melaninogenica (p = 0.0002), Lachnoanaerobaculum saburreum (p < 0.0001), and Streptococcus anginosus (p = 0.0020). Streptococcus intermedia (p = 0.0042), P.nodatum (p = 0.0022), P. micra (p = 0.0002), Treponema denticola (p = 0.0045), L.saburreum (p = 0.0267), P.melaninogenica (p = 0.0017), Campylobacter rectus (p = 0.0020), and T.socranskii (p = 0.0002) were higher; Aggregatibacter actinomycetemcomitans (p = 0.0072) was lower in deep pockets in the PA+PD compared to PD. Schaalia odontolytica (p = 0.0351) and A.actinomycetemcomitans (p = 0.002) were lower; C.rectus (p = 0.0002), P. micra (p = 0065), Streptococcus constellatus (p = 0.0151), T.denticola (p = 0.0141), P.melaninogenica (p = 0.0057), and T.socranskii (p = 0.0316) were higher in shallow pockets in the PA+PD. Diversity decreased in PD (p = 0.001) and PA+PD (p = 0.026) compared to control, with minimal differences in alpha and beta diversities among PD and PA+PD based on NGS results.

Conclusion

These data demonstrated that Parkinson’s disease modifies PD-associated subgingival microbiome.

KEYWORDS:

• Checkerboard
• microbiota
• Parkinson’s disease
• pathogenesis
• periodontitis
• microbiome

Acknowledgments

This research was supported by a grant from the NIH/NIA (R01AG062496 to A. Kantarci).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The next-generation sequencing data for this study are available at https://microbiome.forsyth.org/ftp/fomc/FOMC7142_full/REPORT.html.

Supplementary Material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/20002297.2023.2250650

Additional information

Funding

The work was supported by the National Institutes of Health [R01AG062496].