Role of Dectin-1 in immune response of macrophages induced by Fonsecaea monophora wild strain and melanin-deficient mutant strain

ABSTRACT

Chromoblastomycosis is a chronic granulomatous subcutaneous fungal disease caused mainly by Fonsecaea monophora in southern China. Melanin is an important virulence factor in wild strain (Mel+), and the strains lack of the polyketide synthase gene is a melanin-deficient mutant strain (Mel-). We investigated the effect of melanin in F. monophora on Dectin-1 receptor-mediated immune responses in macrophages. Conidia and tiny hyphae of Mel+ and Mel- were co-cultured with THP-1 macrophages expressing normal or low levels of Dectin-1. Compare the killing rate, phagocytosis rate, and expression levels of the inflammatory cytokines tumour necrosis factor-α, interleukin-1β, interleukin-6, and nitric oxide in each group. The results showed that the killing rate, phagocytosis rate, and pro-inflammatory factor levels of Mel+ infected macrophages with normal expression of Dectin-1 were lower than those of Mel-. And the knockdown of Dectin-1 inhibited the phagocytic rate, killing rate, and proinflammatory factor expression in macrophages infected with Mel+ and Mel-. And there was no significant difference in the above indexes between Mel+ and Mel- groups in Dectin-1 knockdown macrophages. In summary, the study reveals that melanin of F. monophora inhibits the immune response effect of the host by hindering its binding to Dectin-1 on the surface of macrophage, which may lead to persistent fungal infections.

KEYWORDS:

• Fonsecaea monophora
• melanin
• Dectin-1
• macrophage
• phagocytosis
• cytokine

Disclosure statement

No potential conflict of interest was reported by the author(s).

Author contributions

Jiaojiao Zhong, Junmin Zhang, and Jing Zhang designed the experiment; Jiaojiao Zhong and Wenying Cai conducted the experiment; Junmin Zhang, Xiqing Li, and Jianchi Ma revised and reviewed the article.

Supplemental data

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21501203.2023.2249010.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (82002121).