https://orcid.org/0000-0003-1893-1869
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ABSTRACT
Rock-inhabiting fungi (RIF) are slow-growing microorganisms that inhabit rocks and exhibit exceptional stress tolerance owing to their thick melanised cell walls. This study reports the identification of a novel rock-inhabiting fungus, Cladophialophora brunneola sp. nov. which was isolated from a karst landform in Guizhou, China, using a combination of morphological and phylogenetic analyses. The genome of C. brunneola was sequenced and assembled, with a total size of approximately 33.8 Mb, encoding 14,168 proteins and yielding an N50 length of 1.88 Mb. C. brunneola possessed a larger proportion of species-specific genes, and phylogenomic analysis positioned it in an early diverged lineage within Chaetothyriales. In comparison to non-RIF, C. brunneola displayed reduction in carbohydrate-active enzyme families (CAZymes) and secondary metabolite biosynthetic gene clusters (BGCs). Transcriptome analysis conducted under PEG-induced drought stress revealed elevated expression levels of genes associated with melanin synthesis pathways, cell wall biosynthesis, and lipid metabolism. This study contributes to our understanding of the genomic evolution and polyextremotolerance exhibited by rock-inhabiting fungi.
Acknowledgments
The author thank Dr. Baosong Chen (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China) for kindly help in melanin extraction.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The sequencing data are available at NCBI BioProject ID PRJNA952342.
Supplementary material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/21501203.2023.2256764