miR136 regulates proliferation and differentiation of small tail han sheep preadipocytes

ABSTRACT

Low meat performance is the defect of Small Tail Han sheep. Intramuscular fat affects meat quality and largely determined by adipogenesis. In previous study, miR136 was showed one of differentially expressed microRNAs between preadipocytes and mature adipocytes of Small Tail Han sheep but its role in adipogenesis is still not elucidated. Here, we investigated the effect of miR136 on adipogenesis and the underlying mechanism. qPCR data showed that miR136 level increased with preadipocytes proliferation while declined with preadipocytes differentiation. Moreover, miR136 mimics blocked lipid droplet formation, reduced lipid content and triglyceride accumulation while miR136 inhibitor showed the opposite effects, revealing that miR136 promoted preadipocytes proliferation but inhibited preadipocytes differentiation. Bioinformatics and biochemical validation manifested that PPARGC1B was a target of miR136. Furthermore, miR136 mimics decreased PPARγ and C/EBPα expression accompanied by PPARGC1B expression descending. Reverse effects were observed with miR136 inhibitor. Besides, overexpression of miR136 elevated IGF1 expression. Collectively, our data first exhibited a regulatory role of miR136 in adipogenesis, which is promoting preadipocytes proliferation through elevating IGF1 expression while inhibiting preadipocytes differentiation through targeting PPARGC1B and further declined PPARγ and C/EBPα expression. The modulation of PPARGC1B by miR136 may provide a new potential target for increasing intramuscular fat.

KEYWORDS:

• miR136
• differentiation
• proliferation
• sheep preadipocytes
• intramuscular fat

Acknowledgments

This work was supported by Health Commission of Henan Province (LHGJ20210766) and Zhengzhou Central Hospital Affiliated to Zhengzhou University (KYQDJJ2021002).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are available from the corresponding author [email protected] upon reasonable request.

Author contributions

Conceptualization, Luo Man, Wang lin, Xiao Cheng and Li Hongjuan; methodology, Luo Man, Wang Lin, and Xiao Cheng; validation, Zhou Mengsi and Li Minghui; writing—original draft preparation, Luo Man, Wang Lin and Xiao Cheng; writing—review and editing, Li Hongjuan and Wang Lin; visualization, Luo Man, Xiao Cheng and Zhou Mengsi; funding acquisition, Luo Man. All authors have read and agreed to the published version of the manuscript.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21623945.2023.2173966.

Additional information

Funding

This work was supported by Health Commission of Henan Province (LHGJ20210766) and Zhengzhou Central Hospital Affiliated to Zhengzhou University (KYQDJJ2021002).