TRAF6 promotes osteogenesis in ADSCs through Raf-Erk-Merk-Hif1-a pathway

ABSTRACT

Critical-size defects (CSDs) are challenging oral clinical issues that need to be solved. Adipose-derived mesenchymal stem cells (ADSCs) and gene therapy offer a new target to solve these issues. Consequently, ADSCs attract more and more attention because of advantages such as easy obtainability and no ethical concerns. TNF receptor-associated factor 6 (TRAF6) is a significant binding protein both of tumour necrosis factor superfamily and of the toll/interleukin-1 receptor superfamily. Evidence is accumulating that TRAF6 inhibited osteoclast formation and promoted the proliferation of multiple myeloma cell lines and bone resorption. Here, we reported that overexpression of TRAF6 enhanced the proliferation, migration and osteogenesis of ADSCs through Raf-Erk-Merk-Hif1a pathway. Cell sheet of ADSCs combined with TRAF6 accelerated the healing of CSDs. In a word, TRAF6 enhanced osteogenesis, migration and proliferation through Raf-Erk-Merk-Hif1a pathway.

KEYWORDS:

• ADSCs
• TRAF6
• Hif1a
• Osteogenesis

Abbreviations

ADSCS, adipose-derived stem cells; CSD, critical-size defects; TRAF6, TNF-receptor-associated factor 6; TNF, tumour necrosis factor; TIR, toll/IL-1 receptor; BV/TV, bone volume/tissue volume; BS/TV, bone surface area tissue volume ratio; HE, hematoxylin and eosin; VG, Van Gieson; TB, toluidine blue

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

The data that support the findings of this study are openly available in Adipocyte at URL.

Author contributions

Liuxiangdong carried out all the experiments, collection and analysis of data, and wrote the manuscript. Zhengjiang and Songshuang performed experimental guidance and data analysis. Chenzijun, Wangyuxi and Zhangsijia contributed to collection of ADSCs. Luqn and Songyingliang conceived and designed the study. All authors read and approved the final manuscript.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21623945.2023.2193280.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (82170991).