Nitric oxide is involved in regulating cytochrome P450 gene family and mitochondrial related genes of Pleurotus eryngii in response to cadmium stress: preliminary expression patterns analysis

ABSTRACT

  Nitric oxide (NO) plays an important role in fungal response to abiotic stress, but little is known about whether or not NO is involved in regulating cytochrome P450 (CYP) gene family and mitochondrial related genes (MRG) of Pleurotus eryngii in response to cadmium (Cd) stress. In this study, 125 CYP members and 127 differentially expressed MRG were identified. The analysis of gene length and exon number revealed the complexity of CYP gene family. After adding exogenous NO, the expression patterns showed that PleCYP2 and 4-hydroxybenzoate polyprenyltransferase were significantly up-regulated, the functions of membrane and membrane components were enhanced, and the mitochondrial splicing apparatus component gene was activated, which might be the potential mechanism of NO regulating P. eryngii resistance to Cd stress. This study lays a foundation for further revealing the mechanism of NO in regulating P. eryngii response to abiotic stress and promotes the potential application in fungal cultivation.

KEYWORDS:

• Pleurotus eryngii
• cadmium
• nitric oxide
• cytochrome P450
• mitochondrial related genes
• expression patterns

Author contributions

Conceived and designed experiments: C.Z. and Q. L. Performed the experiments: C. Z. and Q.L. Analyzed the data: Q.L. Writing-Original Draft: C.Z. Review & Editing: H. S., L. Z., J. L., T. L., L.C., and Z. C. All authors read and approved the final manuscript.

Disclosure statement

The authors declare that they have no conflicts of interest .

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (grant No. 42107233, 22006004), the Key Research Project of Sichuan Science and Technology Department, China (grant No. 2022YFS0498)