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Abstract:
While it is well known that the cytoskeleton plays a fundamental role in maintaining cell shape, performing cell division, and intracellular transport, its spatiotemporal dynamics are insufficiently understood. The dispersion relation, which is fundamental for understanding the connection between spatial and temporal scales of a dynamic system, was employed here for the first time to study the activity of actin and microtubules. Using green fluorescence protein for time-lapse imaging of the cytoskeleton, we showed that the dispersion relation can distinguish between diffusive and active transport of actin and microtubule filaments. Our analysis revealed that along the filaments, the transport was deterministic, as one might expect as the result of the active polymerization process, while across the filaments diffusion was dominant. Furthermore, using drugs to block the polymerization–depolymerization of both actin and microtubules, we measured that the transport immediately became diffusive, as expected. However, unexpectedly, our results indicated that within a few minutes from blocking its polymerization, actin recovered an active transport component. This deterministic component vanished upon treatment with nocodazole, indicating that fragments of actin were actively transported along microtubules. Because it provides information over broad temporal and spatial scales, this approach promises to provide a new window into the active processes associated with live cells.
Acknowledgment
This research was supported by the National Science Foundation: CBET 0846660 CAREER (GP), CBET 1040462 MRI (GP), CBET-0939511 STC (GP and YW), NIH HL098472 (YW), and NSF DMR-1006162 (AJL).
Disclosure
The authors report no conflicts of interest in this work.