Soyghurt Potentially Controls the Level of sFlt1 and PLGF in Preeclampsia Maternal Serum-Induced Placental Trophoblast Cell in vitro

Abstract

Purpose

To observe the effect of soya yoghurt (Soyghurt), which is high in flavonoid substance, on the expression of preeclampsia biomarkers (sFLT-1 and PLGF) on preeclampsia serum-induced trophoblast primary cell culture isolated from placental tissue.

Methods

The trophoblast primary culture was induced by preeclampsia serum (10%). The Soyghurt treatment was performed with 2.5%, 5%, and 7.5% Soyghurt supernatant concentrations in culture media. The expression of preeclampsia markers, sFLT-1 and PLGF, were evaluated using ELISA.

Results

Expression of sFLT-1 on preeclampsia-induced cell culture treated with Soyghurt was significantly lowered compared to the untreated group (p<0.01). However, no significant difference was observed in the PLGF levels of all groups induced by preeclampsia serum (p>0.05).

Conclusion

This study demonstrates the potential effect of Soyghurt’s in balancing preeclampsia marker expression by inhibiting the expression of sFLT-1 in preeclampsia serum -induced trophoblast cells.

Keywords:

• preeclampsia
• PLGF
• sFLT-1
• soya yoghurt
• trophoblast

Acknowledgments

This study was conducted at the Cell Culture and Cytogenetic Laboratory, Faculty of Medicine, Universitas Padjadjaran, Indonesia. We would like to appreciate the laboratory technicians for their technical assistance during the experimental research.

Author Contributions

All the authors made substantial and proportional contributions to the design, conducting research, acquisition of data, and data analysis. All authors took part in drafting the article or critically revising for important intellectual content. All authors agreed to submit to this journal, given final approval of the version to be published and agreed to be accountable for all aspects of the work.

Disclosure

The authors report no conflicts of interest in this work.

Additional information

Funding

This work was supported by Internal Grant, Universitas Padjadjaran, West Java, Indonesia.