The Effect of Pre-Electroacupuncture on Nociceptive Discharges of Spinal Wide Dynamic Range Neurons in Rat

Abstract

Purpose

Spinal wide dynamic range (WDR) neurons are well studied in pain models and they play critical roles in regulating nociception. Evidence has started to accumulate that acupuncture produces a good analgesic effect via activating different primary fibers with distinct intensities. The purpose of the present study was to compare the distinct intensities of pre-electroacupuncture (pre-EA) at local muscular receptive fields (RFs), adjacent or contralateral non-RFs regulating the nociceptive discharges of spinal WDR neurons evoked by hypertonic saline (HS).

Materials and Methods

Spinal segments of electrophysiological recording were identified by neural tracers applied at the left gastrocnemius muscle. The thresholds of Aβ (T_Aβ), Aδ (T_Aδ) and C (T_C) components of WDR neurons were measured to determine the intensity of pre-EA by extracellular recording. The discharges of WDR neurons induced by distinct intensities of pre-EA and 200 µL HS (6%) injection in left gastrocnemius muscle of rats were observed by extracellular recording.

Results

The spinal segments of WDR neurons were confirmed in lumbar (L)5–6 area according to the projective segments of dorsal root ganglion. T_Aβ, T_Aδ and T_C of WDR neurons was determined to be 0.5, 1, and 2 mA, respectively. The pre-EA with intensities of T_Aβ (P < 0.05), T_Aδ (P < 0.05), T_C (P < 0.05) or 2T_C (P < 0.01) at ipsilateral adjacent non-RFs significantly reduced the discharges of WDR neurons, while at local RFs only pre-EA of T_Aδ (P < 0.05), T_C (P < 0.05) and 2T_C (P < 0.01) could inhibit the nociceptive discharges. In addition, intensity of pre-EA at contralateral non-RFs should reach at least T_C to effectively inhibit the firing rates of WDR neurons (P < 0.01).

Conclusion

Pre-EA could suppress nociceptive discharges of WDR neurons and the inhibitory effects were dependent on the distinct intensities and locations of stimulation.

Keywords:

• pre-electroacupuncture
• wide dynamic range neurons
• muscular receptive fields
• intensity
• hypertonic saline

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (82130122, 81973964). The experiment platform was provided by Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.

Disclosure

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.