Biocidal Efficacies of Contact Lens Disinfecting Solutions Against International Organization for Standardization (ISO) Compendial Organisms

Abstract

Purpose

This study was conducted to evaluate and compare the in vitro disinfection efficacies of six commercial lens cleaning and disinfecting products for planned replacement soft contact lenses.

Methods

Disinfection efficacies of five multi-purpose solutions (MPSs) and one hydrogen peroxide solution (HPS) as control were evaluated in the presence of organic soil according to the International Organization for Standardization (ISO, Geneva, Switzerland) ISO 14729 stand-alone test protocol. The five specified compendial organisms, three bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, and Serratia marcescens) and two fungi (Candida albicans and Fusarium solani) were incubated with each solution under standard conditions, after which microbes were recovered and quantified.

Results

Each of the solutions evaluated met or exceeded the standard’s primary criteria (3-log reduction of bacteria and 1-log reduction of fungi) after incubation for the manufacturer-recommended soaking time, except for COMPLETE MPS, which achieved only 0.4 ± 0.1 average log reduction for C. albicans. However, differences in efficacy between the solutions were noted. Average log reduction across all microbes for Biotrue Hydration Plus (4.6 ± 0.1) was comparable to that for CLEAR CARE PLUS HPS (4.3 ± 0.1) and greater than those for OPTI-FREE puremoist (3.6 ± 0.1), OPTI-FREE Replenish (4.0 ± 0.2), ACUVUE RevitaLens (3.9 ± 0.03), and COMPLETE MPS (3.6 ± 0.1). Biotrue Hydration Plus was especially effective at reducing the population of C. albicans (4.2 ± 0.7-log reduction).

Conclusion

Products marketed for planned replacement soft CL disinfection generally meet the ISO 14729 standard’s primary criteria for reducing populations of compendial organisms, with larger differences between solutions noted with C. albicans.

Keywords:

• multi-purpose solution
• MPS
• hydrogen peroxide solution
• HPS
• disinfection
• myristamidopropyl dimethylamine
• MAPD
• polyhexamethylene biguanide
• PHMB
• ISO 14729

Data Sharing Statement

All relevant data are within the manuscript. Clarification requests around the manuscript and its data can be made to the corresponding author.

Acknowledgments

The authors thank William Reindel and Marjorie Rah (both Bausch & Lomb Incorporated) for thoughtful discussions. Medical writing/editorial support was provided by Joseph Chinn (J Chinn LLC, Lafayette CO) and funded by the study sponsor.

The abstracts of this paper were presented at the Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting, May 1–4, 2022, as a poster presentation (Board A0240), and the American Optometric Association (AOA)’s Optometry’s Meeting, June 15–18, 2022, as a poster presentation (Submission ID 1681). The ARVO poster abstract was published online as follows: Corwin-Buell J, Callahan D, McGrath D, Millard K. Disinfection efficacy testing of a triple disinfectant system in an investigational multi-purpose solution challenged against the five bacterial and fungal compendial organisms. Invest Ophthal Vis Sci. 2022; 63:542-A0240, and is available for download from https://iovs.arvojournals.org/article.aspx?articleid=2779577. [Cited 01 Sep 2023].

Author Contributions

All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

All the authors are employees of Bausch & Lomb Incorporated.

Additional information

Funding

This work was funded by Bausch & Lomb Incorporated.