https://orcid.org/0000-0002-9504-7914
Abstract
3D bioprinting is a powerful enabling technology for the automated fabrication of biomimetic constructs for skin modelling and repair with high resolution and reproducibility. Bioinks, which often comprise the combination of cells and printable biomaterials, are essential for bioprinting 3D cell-laden constructs emulating the architecture, composition and function of native skin. Tissue- and organ-specific decellularized extracellular matrix (dECM) materials are assuming a significant role in bioink design owing their capacity to provide native biophysical and biochemical signals that can elicit distinct cellular responses toward tissue development. Herein, we discuss the rational design of dECM-based bioinks, focusing on processing methods to obtain dECM as well as on bioprinting strategies to engineer 3D constructs for applications in wound healing and in vitro skin modelling.
Acknowledgments
The authors acknowledge the BE@T – Bioeconomy for Textiles and Apparel, investment TC-C12-i01 – Sustainable Bioeconomy, funded through the Recovery and Resilience Program (PRR) and the FCT through the project UID/Multi/50016/2020. JB Costa, IV Silva and R Presa are grateful to FCT for the individual Junior Researcher contract (doi.org/10.54499/2022.02781.CEECIND/CP1745/CT0001), and Doctoral Research Grants (2021.05919.BD) and (2022.13383.BD), respectively.
Financial disclosure
This work was supported by FEDER – Fundo Europeu de Desenvolvimento Regional funds through COMPETE 2020 – Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and Portuguese funds via FCT – Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the projects doi.org/10.54499/2022.06048.PTDC and UID/BIM/04293/2020, as well as by the project IBEROS+ (0072_IBEROS_MAIS_1_E) via “Interreg VI A Espana – Portugal (POCTEP) 2021-2027”. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
Competing interests disclosure
The authors have no competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, stock ownership or options and expert testimony.
Writing disclosure
No writing assistance was utilized in the production of this manuscript.