Chemically Synthesized Ciprofloxacin-PEG-FeO Nanotherapeutic Exhibits Strong Antibacterial and Controlled Cytotoxic Effects

Abstract

Aim: To develop a biocompatible conjugated ciprofloxacin-PEG-FeO nanodelivery system with increased efficacy of available therapeutics in a controlled manner. Materials & methods: FeO nanoparticles were synthesized by chemical and biological methods and modified as ciprofloxacin-PEG-FeO nanoformulations. After initial antibacterial and cytotoxicity studies, the effective and biocompatible nanoformulations was further fabricated as nanotherapeutics for in vivo studies in mouse models. Results: Chemically synthesized ciprofloxacin-PEG-FeO nanoformulations demonstrated boosted antibacterial activity against clinically isolated bacterial strains. Nanoformulations were also found to be compatible with baby hamster kidney 21 cells and red blood cells. In in vivo studies, nanotherapeutic showed wound-healing effects with eradication of Staphylococcus aureus infection. Conclusion: The investigations indicate that the developed nanotherapeutic can eradicate localized infections and enhance wound healing with controlled cytotoxicity.

Tweetable abstract

A ciprofloxacin-PEG-FeO nanodrug delivery system was developed, which showed enhanced antibiotic efficacy with a broad range of effects and controlled cytotoxicity in in vitro and in vivo experiments.

Keywords: :

• antibacterial
• CIP-PEG-FeO nanoformulation
• controlled cytotoxicity
• mouse model
• therapeutic agents

Supplementary data

Author contributions

Conceptualization: S Nisa and Hussan. Methodology: S Nisa and Hussan. Software: Hussan Validation: S Nisa and M Zia. Formal analysis: Hussan. Investigation: S Nisa and M Zia. Resources: M Zia. Data interpretation: Hussan. Writing: Hussan. Review and editing: S Nisa and SA Bano. Visualization: S Nisa and M Zia. Supervision: S Nisa and M Zia. Project administration: S Nisa, SA Bano and M Zia. All authors have read and agreed to the final version of the manuscript.

Financial disclosure

The authors have no financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or royalties.

Competing interests disclosure

The authors have no competing interests or relevant affiliations with any organization or entity with an interest in or conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or royalties.

Writing disclosure

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The present study was approved by the departmental or institutional review board and ethical review board of the University of Haripur (Haripur, Pakistan) with registration no. (F20-1986-MIC-PhD[Micro]/UOH) and NIH ethical letter (F.1-5/ERC/2021).