Nanoparticles Integrated with Mild Photothermal Therapy and Oxaliplatin for Tumor cHemotherapy and Immunotherapy

Abstract

Aims: Preparation and evaluation of nanoparticles for tumor chemotherapy and immunotherapy mild photothermal therapy and oxaliplatin. Methods: The double emulsion method was used for nanoparticle preparations. Polydopamine was deposited on the surface, which was further modified with folic acid. Cytotoxicity assays were carried out by cell counting kit-8. In vivo antitumor assays were carried out on 4T1 tumor-bearing mice. Results: The nanoparticles exhibited a 190 nm-diameter pomegranate-like sphere, which could increase temperature to 43–46°C. In vivo distribution showed enhanced accumulation. The nanoparticles generated stronger immunogenic cell death effects. By stimulating the maturation of dendritic cells, mild photothermal therapy combined with oxaliplatin significantly increased the antitumor effect by a direct killing effect and activation of immunotherapy. Conclusion: This study provided a promising strategy of combination therapy for tumors.

Keywords: :

• albumin nanoparticles
• immunogenic cell death
• mild hyperthermia
• oxaliplatin
• polydopamine nanoparticles
• tumor-targeting

Supplementary data

Financial disclosure

This work was financially supported by the Talent Initiation Program of Chengdu University (2081922007). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Competing interests disclosure

The authors have no competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or royalties.

Writing disclosure

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

Female BALB/c mice (20 ± 2 g) were purchased from Dashuo Biotechnology Co. Ltd (Chengdu, China) and maintained under standard housing conditions. All animal experiments were performed in accordance with the rules of the ethics committee of Chengdu University. The experiments minimized animal suffering and used fewer mice.