Acrylamide and glycidamide in plasma of diabetic and non-diabetic rats, a comparative toxicokinetic study

Abstract

Acrylamide is converted to glycidamide as a reactive metabolite by the monooxygenase isozyme CYP2E1. Since the latter is known to be induced in diabetic patients, increased acrylamide toxicity in such patients is suspected.

Differences in acrylamide toxicokinetics in non-diabetic and diabetic rats receiving acrylamide (50 mg/kg) orally or via i.p. injection were investigated in this report. Blood was collected at various time points, acrylamide and glycidamide in plasma were determined by reversed-phase high-performance liquid chromatography, and the data were analyzed for toxicokinetic parameters using the proper software.

Mean maximum plasma concentration, the apparent clearance, and area under the curve in non-diabetic rats were significantly higher than in diabetics, an important fact to be considered in xenobiotic exposure of diabetic individuals.

Keywords:

• Diabetes mellitus
• acrylamide
• glycidamide
• CYP2E1
• toxicokinetics
• chromatography

Acknowledgments

The authors are thankful to Mashhad University of Medical Sciences for financial support. The results in this paper were part of the thesis of Asieh Karimani.

Author contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Amir Hooshang Mohammadpour, Gholamreza Karimi, and Asieh Karimani. The first draft of the manuscript was written by Asieh Karimani and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors are thankful to Mashhad University of Medical Sciences for financial support.