Talaromyces purpurogenus MRS-F13 catalyzed biotransformation of (-)-verbenone to (-)-10-hydroxyverbenone and their anti-inflammatory activity profile

Abstract

Verbenone is a natural monoterpene present as an essential component in rosemary oil from Rosmarinus officinalis, Verbena triphylla and Eucalyptus globule. Microbial biotransformation of monoterpenoids to value-added products has immense industrial potential. This study involves the biotransformation of (-)-verbenone to (-)-10-hydroxyverbenone by a fungus i.e. Talaromyces purpurogenus strain MRS-F13 in fermentation broth during the exponential phase. Biotransformation reaction has been successfully demonstrated at 15 L scale in the bioreactor with 70% conversion within 96 h. The anti-inflammatory activity investigations revealed that (-)-verbenone and its biotransformed product exhibited moderate inhibition of TNF-α and nitric oxide, whereas (-)-10-hydroxyverbenone presented an improved anti-oxidant activity.

Highlights

• Talaromyces purpurogenus catalysed biotransformation of (-)-verbenone to (-)-10-hydroxyverbenone has been demonstrated.

• Approximately 70% bioconversion was obtained in 96 h in submerged culture at 15L bioreactor scale.

• Biotransformed product (-)-10-hydroxyverbenone has shown improved anti-inflammatory activity without cytotoxicity.

Keywords:

• Biotransformation
• Talaromyces purpurogenus
• (-)-verbenone
• (-)-10- hydroxyverbenone
• anti-inflammatory activity

Acknowledgements

Authors are thankful to Director, CSIR-IIIM for his interest in this study. Financial support from CSIR Aroma Mission (HCP-007) is gratefully acknowledged. RSM and PK thank ICMR and CSIR for their respective fellowships. The manuscript bears Institutional publication no. CSIR-IIIM/IPR/00188.

Ethical approval

This article does not include any studies conducted by any of the authors with human or animal participants.

Author contributions

RSM carried out most of the experiments and wrote the manuscript, DK helped in optimization experiments, isolation of compounds & formatting of manuscript, PK executed anti-inflammatory activity experiments, AK carried out analytical experiments, AB provided technical supervision for anti-inflammatory experiments, SM designed anti-inflammatory experiments, data analysis, interpretation and write-up, PLS supervised isolation and characterized the compounds, ZA reviewed overall anti-inflammatory activity and write-up, AC conceptualized, coordinated, supervised the entire work and corrected the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data relevant to the manuscript is available with the corresponding author. Please Contact author for data requests. Sequence file for the sample used in this study have been deposited at NCBI with accession no. MN907642.

Additional information

Funding

The funding for the reported work was received from CSIR-Aroma Mission (HCP-007).