Enzymatic degradation and metabolic pathway of acid blue 129 dye by crude laccase from newly isolated Trametes hirsuta EDN 082

Abstract

Crude laccase from Trametes hirsuta EDN 082 was used in this study for the decolorization of anthraquinone and azo dyes. Characterization of degradation products from one selected dye, Acid Blue 129 (AB129), was investigated using liquid chromatography high-resolution mass spectrometry (LC-HRMS). The results showed that laccase from T. hirsuta EDN 082 (0.25 U/mL dyes) was able to decolourize AB 129, AB 113, RBBR, AB 25, RB4, and RB5 dyes with a decolorization efficiency of 88%, 73%, 71%, 72%, 42%, and 6%, respectively for 7 h. During the degradation of AB129 dye, nine metabolites were identified and characterized by electrospray ionization–liquid chromatography–mass spectrometry Quadrupole-Time of flight (ESI-LCMS-Q-TOF) analysis. Evaluation of peak area showed decreasing of the two metabolites, 1-amino-4-hydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid (D2) and 2,4,6-trimethylaniline (D4) during 7 h reaction indicated these two compounds become the centre of the degradation after cleaving the AB129 dye, and from these results, metabolic pathways were proposed. This study offers the potency of laccase from T. hirsuta EDN 082 for bioremediation of synthetic dyes in the fields.

Keywords:

• Decolorization
• laccase
• liquid chromatography–mass spectrometry
• metabolic pathway
• textile dyes

Acknowledgements

This work was supported by National Priority Program of Deputy of Life Sciences, National Research and Innovation Agency (BRIN). The authors acknowledge the facilities, and the scientific and technical assistance of the Integrated Laboratory of Bioproducts at National Research and Innovation Agency (BRIN). We also thank Shimadzu (Asia Pacific) and PT Ditek Jaya for assisting ESI-LCMS-Q-TOF analysis.

Author contribution

All the authors are main contributors. Dede Heri Yuli Yanto: conceptualization, supervision, methodology, funding acquisition, formal analysis, writing. Sita Heris Anita: investigation, formal analysis, writing. Nissa Nurfajrin Solihat: investigation, formal analysis, writing.

Disclosure statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.