Immobilization of Trametes trogii laccase on polyvinylpyrrolidone-coated magnetic nanoparticles for biocatalytic degradation of textile dyes

Abstract

High cost and low operational stability are the most important challenges limiting the possible use of laccase in the removal of textile dyes. To overcome these challenges, in this study, polyvinylpyrrolidone (PVP)-coated magnetic nanoparticles (MNPs) were produced and characterized. To our knowledge, this is the first study to explore the feasibility of immobilizing a Trametes trogii laccase enzyme on Fe₃O₄/PVP MNPs. The characterization of samples and the successful immobilization of laccase were verified by characterization methods. Besides, the biochemical properties and stability of the Fe₃O₄/PVP/Lac were evaluated in terms of optimum pH, optimum temperature, thermostability, thermodynamic and kinetic parameters, storage stability, operational stability, and decolorization efficiency of two different textile dyes. The optimum activities were recorded at pH 2.5 °C and 30 °C. The Fe₃O₄/PVP/Lac displayed remarkable thermal stability and activation energy for denaturation, enthalpy, Gibbs free energy, and entropy results confirmed the enhanced stability of Fe₃O₄/PVP/Lac against high temperatures. Meanwhile, the Fe₃O₄/PVP/Lac retained about 58% of its original activity after seven consecutive reuses, while it retained up to 25% of its original activity after 28 d of storage at room temperature. K_m and V_max for the Fe₃O₄/PVP/Lac were calculated to be 126 µM and 211 µmol/min, respectively. Finally, after 8 and 6 cycles of repeated use, the Fe₃O₄/PVP/Lac still decolorized 32.34% and 32.23% of Remazol Brilliant Blue R (RBBR) and Indigo Carmine (IC), respectively. As envisioned, this study suggests a promising way to solve the problems of high price and poor operational stability of the enzyme during biocatalytic decolorization of textile dyes in wastewaters.

Keywords:

• Magnetic nanoparticles
• polyvinylpyrrolidone
• laccase immobilization
• Remazol Brilliant Blue R
• Indigo Carmine
• dye decolorization

Acknowledgements

The authors expressed their gratitude to İnönü University.

Disclosure statement

The authors express report no declarations of interest.

Additional information

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.