Triangularia manubriata sp. Nov.: A Novel Fungal Species Belonging to the Family Podosporaceae Isolated from Soil in Korea

Figures & data

Table 1. GenBank accession numbers of the sequences used for phylogenetic analyses in this study.

Species	Strain	GenBank accession number
		ITS	LSU	RPB2	TUB2
Podospora fimicola	CBS 482.64	MK926862	MK926862	MK876824	MK926962
Triangularia allahabadensis	CBS 724.68^T	MK926865	MK926865	MK876827	MK926965
Triangularia anserina	CBS 433.50	MK926864	MK926864	MK876826	MK926964
Triangularia bambusae	CBS 352.33^T	MK926868	MK926868	MK876830	MK926968
Triangularia backusii	CBS 539.89^T	MK926866	MK926866	MK876828	MK926966
Triangularia backusii	CBS 106.77	MK926867	MK926867	MK876829	MK926967
Triangularia longicaudata	FMR 12365	MT784141	KP981448	KP981631	KP981574
Triangularia longicaudata	FMR 12782	MT784142	KP981449	KP981632	KP981575
Triangularia manubriata sp. nov.	KNUF-21-020^T	OR727458	OR727459	OR729840	OR729841
Triangularia phialophoroides	CBS 301.90^T	MK926871	MK926871	MK876833	MK926971
Triangularia setosa	CBS 311.58	MK926872	MK926872	MK876834	MK926972
Triangularia setosa	CBS 369.59	MK926873	MK926873	MK876835	MK926973
Triangularia verruculosa	CBS 148.77	MK926874	MK926874	MK876836	MK926974

ITS: internal transcribed spacer; LSU: large subunit of 28S rRNA; RPB2: RNA polymerase subunit II; TUB2: beta-tubulin.Bold values indicate new strains of the genus Triangularia.

Figure 1. Cultural and morphological characteristics of Triangularia manubriata KNUF-21-020. Cultures were grown at 25 °C for seven days. (A–D) Front and reverse view of the colony on PDA, OA, MEA, and PCA, respectively; (E, F) ascomata produced on PDA; (G) asci; (H) premature (hyaline) and matured (melanized) ascospore. Scale bars: E, F = 50 µm, G, H = 20 µm.

Table 2. Morphological characteristics of Triangularia manubriata (KNUF-21-020^T) and comparison with the closest species of Triangularia.

Characteristics		T. manubriata KNUF-21-020^Ta	T. setosa CBS 311.58^b	T. bambusae CBS 325.33^Tb
Colony	Color	OA: white, buff in center and layer on edge by aerial mycelium, absence of exaduate and ascomata	OA: vinaceous, without aerial mycelium, no exaduate, black dot-like ascomata	OA: white, layer aerial mycelium, salmon to peach-colored exudate, black dot-like ascomata
		PCA: white, layer in center and buff on edge by aerial mycelium, absence of exaduate and ascomata	PCA: uncolored, crenate edge, without aerial mycelium	PCA: white, thin layer of aerial mycelium, absence of exaduate and ascomata
		MEA: white, layer in center and buff on edge by aerial mycelium, absence of exaduate and ascomata	MEA: grey white to vinaceous, buff aerial mycelium, numerous ascomata	MEA: white, thick layer aerial mycelium, fulvous exudate, distributed black dot-like ascomata
	Diameter	OA: 80 mm in 7 d	OA: 24–30 mm in 7 d	OA: 70 mm in 7 d
		PCA: up to 90 mm in 6 d	PCA: 21–27 mm in 7 d	PCA: 70 mm in 7 d
		MEA: up to 90 mm on 6 d	MEA: 18–24 mm in 7 d	MEA: 70 mm in 7 d
Ascospores	Shape and color	Triangular upper cell: obtuse end, olivaceous brown; lower cell: handle-shaped, hyaline	Ellipsoidal upper cell: olivaceous brown; lower cell: handle-shaped, hyaline	Triangular upper cell: aciculiform end, olivaceous brown; lower cell: paler vinaceous buff
	Size	12.0–20.2 × 15.3–25.9 μm	17.0–20.5 × 10.5–12.0 μm	15.5–16.0 × 18.5–20.0 μm
Asci	Shape and color	Superficial, dark brown to black, solitary to aggregated, nonostiolate	Superficial, mouse-gray, solitary, ostiolate	Superficial to semi-immersed, setae, ostiolate, obpyriform

d: days; MEA: malt extract agar; OA: oatmeal agar; PCA: potato carrot agar.

^aFungal strain of this study.

^bSource of description [2].

^TType strain.

Figure 2. Neighbor-joining phylogenetic tree based on a combined dataset of the internal transcribed spacer (ITS) regions and partial large-subunit (LSU), beta-tubulin (TUB2), and RNA polymerase II subunit 2 (RPB2) genes sequences showing the phylogenetic position of the strain KNUF-21-020 among Triangularia species. Bootstrap values greater than 60% (percentage of 1000 replications) are shown at branching points. The strain isolated in this study is in bold. The tree was rooted using Podospora fimicola CBS 482.64^T as an out-group. Bar, 0.02 substitutions per nucleotide position.

Wang XW, Bai FY, Bensch K, et al. Phylogenetic re-evaluation of Thielavia with the introduction of a new family Podosporaceae. Stud Mycol. 2019;93(1):155–252. doi: 10.1016/j.simyco.2019.08.002.

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