388
Views
0
CrossRef citations to date
0
Altmetric
Research Articles

Effects of Environmental and Nutritional Conditions on Mycelium Growth of Three Basidiomycota

, ORCID Icon, &
Pages 124-134 | Received 16 Feb 2024, Accepted 05 Apr 2024, Published online: 25 Apr 2024

Figures & data

Table 1. List of fungal species used in this study.

Figure 1. Top row: milled substrate mixtures of pine bark and cotton fibers in different weight proportions; lower row: (a) raw pine bark; (b) cotton fibers used for this study.

Figure 1. Top row: milled substrate mixtures of pine bark and cotton fibers in different weight proportions; lower row: (a) raw pine bark; (b) cotton fibers used for this study.

Table 2. List of substrate mixtures and additives used in this study.

Table 3. Determined chemical characteristics and calculated C/N ratio of the investigated substrates.

Figure 2. Elemental composition of the substrates I–V in terms of potassium (K), calcium (Ca), magnesium (Mg), phosphorus (P), iron (Fe), sodium (Na), manganese (Mn), zinc (Zn), copper (Cu), nickel (Ni), chromium (Cr), cobalt (Co), and lead (Pb) (mg kg−1 DM); n = 3.

Figure 2. Elemental composition of the substrates I–V in terms of potassium (K), calcium (Ca), magnesium (Mg), phosphorus (P), iron (Fe), sodium (Na), manganese (Mn), zinc (Zn), copper (Cu), nickel (Ni), chromium (Cr), cobalt (Co), and lead (Pb) (mg kg−1 DM); n = 3.

Figure 3. Photos taken of Ganoderma lucidum, Pleurotus ostreatus, and Trametes versicolor after 1, 4, and 7 days of incubation at 26 °C, 80% relative humidity, in the absence of light. Substrate IDs IV and V stand for mixture ratios of 1:3 and 0:4 of pine bark and cotton fiber, respectively.

Figure 3. Photos taken of Ganoderma lucidum, Pleurotus ostreatus, and Trametes versicolor after 1, 4, and 7 days of incubation at 26 °C, 80% relative humidity, in the absence of light. Substrate IDs IV and V stand for mixture ratios of 1:3 and 0:4 of pine bark and cotton fiber, respectively.

Figure 4. Radial expansion (mm d−1) of three Basidiomycota measured over seven days with 0, 5, and 10 vol.% flush of CO2 on the five different substrate mixtures with addition of 0, 2, and 5 wt.% CaCO3. Data were collected for three replicates of the experiment (n = 3) run in parallel. Radius was measured every 24 h in triplicate by the distance from the center of the inoculum to the longest visible hyphae.

Figure 4. Radial expansion (mm d−1) of three Basidiomycota measured over seven days with 0, 5, and 10 vol.% flush of CO2 on the five different substrate mixtures with addition of 0, 2, and 5 wt.% CaCO3. Data were collected for three replicates of the experiment (n = 3) run in parallel. Radius was measured every 24 h in triplicate by the distance from the center of the inoculum to the longest visible hyphae.

Table 4. Effect of substrate, addition of buffer CaCO3 and flush of CO2 on the mycelial growth of three fungi, n = 3, mean ± std.