Antimicrobial Activities of Volatile Components and Various Extracts of Dictyopteris membranaceae. and Cystoseira barbata. from the Coast of Izmir, Turkey

Figures & data

Table 1 Volatile components of D. membranacea. (GC-MS analysis).

Rt (min)	Compound	Area (%)
0.61	3-Allycyclobutane	20.16
11.55	Bicyclo-(2,2,1)-heptadiene	4.07
11.92	6-Butyl-1,4-cycloheptadiene	43.21
20.38	Thiophene-D3	15.72
21.49	(E.)-Farnesene	2.67
	Total	85.83

Table 2 Volatile components of C. barbata. (GC-MS analysis).

Rt (min)	Compound	Area (%)
6.27	Octane	0.65
6.51	Heptane	0.78
6.72	2,4-Dimethyl-1-heptene	1.15
10.13	Undecane	0.74
10.98	Tridecane	0.71
11.23	Tetradecane	0.23
14.65	Pentadecane	1.61
15.35	1-Octene	2.01
15.55	Hexadecane	4.16
15.76	Heptadecane	1.35
15.96	Octadecane	0.74
18.60	Nonadecane	0.94
18.89	Eicosane	5.05
19.06	Heneicosane	1.03
19.32	Phenol, 2,4 bis. (1,1-dimethyl ethyl)	2.74
19.70	Docosane	7.61
19.90	Tricosane	4.43
20.04	Tetracosane	2.11
20.11	Pentacosane	1.99
20.27	Hexacosane	2.32
20.37	Heptacosane	0.61
20.47	Octacosane	1.44
20.55	Nonacosane	0.78
22.89	Triacontane	3.18
23.05	Tritriacontane	0.96
23.75	Tetratriacontane	7.47
24.05	1-Heptadecanamin	6.31
24.26	Pentatriacontane	4.51
	Total	67.61

Rt = retention time.

Table 3 Antimicrobial activity of D. membranacea. and C. barbata. volatile components.

		Inhibition zone (mm)^a.
		0.015 µg/disk		Standard
Microorganism	G	D. membranacea.	C. barbata.	Tob	Nys
S. epidermidis.	+	–	–	7	nt
S. aureus.	+	–	7	16	nt
S. faecalis.	+	–	–	9	nt
B. subtilis.	+	–	–	24	nt
S. typhimurium.	–	–	7	10	nt
P. aeruginosa.	–	7	–	12	nt
E. cloacea.	–	–	–	13	nt
E. coli.	–	–	7	10	nt
C. albicans.		–	–	nt	18

Tob, tobramycin (10 µg/disk); Nys, nystatin (30 µg/disk); nt, not tested; G, Gram reaction; –, no activity.

^a.Zone of inhibition, including the diameter of the filter paper disk (6 mm); mean value of three independent experiments.

Table 4 Antimicrobial activity of D. membranacea. extracts.

				Diameter of zone of inhibition (mm)
	Methanol (mg/disk)			Dichloromethane (mg/disk)			Hexane (mg/disk)			Chloroform (mg/disk)
Microorganism	1 mg	2 mg	4 mg	1 mg	2 mg	4 mg	1 mg	2 mg	4 mg	1 mg	2 mg	4 mg
S. epidermidis.	—	—	—	—	—	—	—	—	8	—	—	—
S. aureus.	—	—	—	—	—	—	11	12	13	—	—	—
S. faecalis.	—	—	—	—	—	—	—	—	—	—	—	—
B. subtilis.	—	—	—	—	—	—	—	—	7	—	—	7
S. typhimurium.	—	—	—	—	—	—	—	7	8	—	—	7
P. aeruginosa.	—	—	—	—	—	—	—	—	—	7	7	8
E. cloacea.	—	—	—	—	—	—	7	—	—	—	—	8
E. coli.	—	—	—	—	—	—	—	7	—	—	—	8
C. albicans.	—	—	9	—	—	—	—	11	16	—	—	—

Table 5 Antimicrobial activity of C. barbata. extracts.

				Diameter of zone of inhibition (mm)
	Methanol (mg/disk)			Dichloromethane (mg/disk)			Hexane (mg/disk)			Chloroform (mg/disk)
Microorganism	1 mg	2 mg	4 mg	1 mg	2 mg	4 mg	1 mg	2 mg	4 mg	1 mg	2 mg	4 mg
S. epidermidis.	—	7	7	—	7	7	7	7	8	7	7	8
S. aureus.	—	—	—	—	—	—	10	12	16	—	7	—
S. faecalis.	—	—	—	—	—	—	—	—	—	—	—	—
B. subtilis.	—	—	—	—	—	—	—	—	—	—	—	7
S. typhimurium.	—	—	—	—	—	—	—	—	—	—	—	8
P. aeruginosa.	—	—	—	—	7	7	7	7	8	—	—	—
E. cloacea.	—	—	—	—	7	7	7	7	8	—	—	—
E. coli.	—	—	—	—	—	—	—	—	—	—	—	—
C. albicans.	—	—	—	—	—	—	—	—	—	—	—	—