Pharmacognostical Evaluation of Cassia angustifolia. Seeds

Figures & data

Figure 1 A twig showing floral characters of the plant.

Figure 2 Macroscopic characters of seed.

Figure 3 SEM view of whole seed.

Figure 4 Surface characters of distal end showing papillae in SEM.

Figure 5 Surface ornamentation of seed through SEM.

Figure 6 Inner cells of seed in lateral view (2000×) through SEM

Figure 7 Cellular structure of seed showing micropyle and cotyledons. Cu, cuticle; Ep, epidermis; Oi, outer integument; Ii, inner integument; Hc, hourglass cells; Vr, vascular regions; Ed, endosperm; Cot, cotyledons; Pc, palisade-like cells; Ms, mesophyll; Ie, inner epidermis.

Figure 8 Cellular structure of seed showing seed coat and endosperm. Cu, cuticle; Ep, epidermis; Oi, outer integument; Ii, inner integument; Hc, hourglass cells; Vr, vascular regions; Ed, endosperm; Cot, cotyledons; Pc, palisade-like cells; Ms, mesophyll; Ie, inner epidermis.

Figure 9 Cellular structure of cotyledon.

Figure 10 Endospermal region in T.S. of seed.

Table 1 Powder studies in seed of C. angustifolia..

Sl. no.		Daylight	UV 254	UV 366
1.	Powder (P) as such	Yellow	Fluorescent yellow	Fluorescent yellow
2.	P + nitrocellulose in amyl acetate	Yellow	Fluorescent yellow	Fluorescent green
3.	P + 1 N NaOH in water	Fluorescent yellow	Dark fluorescent yellow	Fluorescent green
4.	P + 1 N NaOH in nitrocellulose in amyl acetate	Fluorescent yellow	Fluorescent yellow	Fluorescent yellow
5.	P + 1 N HCl	Creamish-yellow	Light fluorescent	Brown
6.	P + 1 N HCl + nitrocellulose in amyl acetate	Orangish-yellow	Fluorescent cream	Fluorescent green
7.	P + 1 N NaOH in methanol	Fluorescent yellow	Fluorescent yellow	Fluorescent green
8.	P + 50% KOH	Creamish-yellow	Dark fluorescent yellow	Grenish fluorescent
9.	P + 50% H2SO4	Fluorescent yellow	Light fluorescent yellow	Fluorescent green
10.	P + 50% HNO3	Creamish-yellow	Fluorescent yellow	Dark-brown with greenish
11.	P + concentration HNO3	Orangish-brown	Fluorescent grid gr gots	Fluorescent green
12.	P + acetic acid	Light brown	Fluorescent green
14.	P + I2 water	Cream	Fluorescent green	Green

Figure 11 Physicochemical parameters of Cassia angustifolia. seeds.

Figure 12 Successive extractive values of Cassia angustifolia. seeds.

Figure 13 HPTLC fingerprint profile of different samples of Cassia angustifolia. seeds procured from different regions (1–4) along with the active constituents sennoside A and B.

Table 2 Percentage concentration of sennoside A and B in seed samples of different geographic regions.

	Dehradun	Hyderabad	Jodhpur	Mumbai
Sennoside A	1.000%	2.134%	1.856%	0.219%
Sennoside B	3.951%	7.446%	8.526%	1.348%