Potential Effect of Acacia arabica. on Peste des Petits Ruminants. Virus Replication

Figures & data

Table 1 Cytotoxicity babul aqueous extract on Vero cells.

BExt concentration (μg/mL)	Total cell count/mL	Viable cell count/mL	Viable cells (%)
50	6.0 × 10^6	6.0 × 10^6	100.00
75	5.8 × 10^6	5.7 × 10^6	98.28
100	5.9 × 10^6	5.8 × 10^6	98.30
150	5.7 × 10^6	5.5 × 10^6	96.49
200	5.0 × 10^6	4.0 × 10^6	80.00
400	5.0 × 10^6	3.7 × 10^6	74.00
500	5.1 × 10^6	2.8 × 10^6	54.90
600	3.2 × lO^6	1.4 × l0^6	43.75
700	3.1 × 10^6	l.O × lO^6	33.33
1000	3.1 × 10^6	l.O × lO^6	33.33

Figure 1 Anti-PPRV activities of BExt in dose-dependent manner.

Figure 2 Agarose gel electrophoresis of RT-PCR products from infected Vero cells with or without BExt using PPRV-specific primers (A) Fl and F2 and (B) NP3a and NP4. (A, B) Lane 1: PPRV grown vero cell without BExt as positive control Lane 2: Uninfected vero cell as negative control. Lane M: 100 bp plus DNA ladder (MBI, Fermentas, USA). (A) Lanes 3, 4 and 5: PPRV grown Vero cell with BExt at concentrations of 100, 150, and 200 μg/mL, respectively. (B) Lanes 3 and 4: PPRV grown Vero cell with BExt at concentrations of 150 and 200 μg/mL, respectively.

Figure 3 Real-time PCR on infected Vero cells with or without BExt using PPRV-specific N-gene primers NP3a and NP4. (A) PCR amplification plot: Green line: PPRV grown without BExt as virus control. Blue line: PPRV grown with 150 μg/mL Bext. Red line: PPRV grown with 200 μg/mL BExt; Gray line: Uninfected Vero cell as negative control. Yellow line: Non-template control. (B) Dissociation curve: Analysis of amplicons derived from melting temperature. (C) Agarose gel electrophoresis of real-time PCR products in 1.5% agarose gel. Lanes 1 and 2: PPRV grown Vero cell with BExt at a concentrations of 200 and 150 μg/mL, respectively, Lane 3: Virus control, Lane 4: Uninfected Vero cell as negative cell control, Lane M: 100 bp plus DNA ladder (MBI, Fermentas).

Figure 4 Effect of viral antigen load on the anti-PPRV activity of BExt on extracellular, intracellular, and total viruses quantified by s-ELISA (A) Adsorption method (indirect). (B) Virucidal method (direct).