Optical-resonator-based biosensing systems: current status and future prospects

Figures & data

Figure 1 (A) Optical whispering gallery modes in spheres. (B) Resonant frequency shift observed during binding. (C) Polarization of biomolecules in evanescent field.

Figure 2 (A) Working principle of a ring resonator. (B) Schematic of solid-phase recombinase polymerase DNA amplification on the resonator.

Notes: Reprinted from Biosens Bioelectron, 2015;73:130–137. Sabaté del Río J, Steylaerts T, Henry OYF, et al. Real-time and label-free ring-resonator monitoring of solid- phase recombinase polymerase amplification.² © 2015 with permission from Elsevier, http://www.journals.elsevier.com/biosensors-and-bioelectronics/.
Abbreviation: IR, infrared.

Figure 3 Dual microring resonator optical system.

Notes: (A) The difference between the tracing and sensor microrings’ wavelengths are captured as power (W₁) before antigen binding. (B) Antigen binding occurs in the sensor microring and the difference in wavelengths are also recorded as power (W₂). Difference in power (W₁– W₂) is proportional to the surface density of the polymer layer covering the sensor microring.

Figure 4 (A) Flow-through microring setup. (B) Comparison of response times using flow-over and flow-through designs.

Figure 5 (A) Overview of entire chip with three rolled-up microtubes. (B) Microtube in SU-8 sockets. (C) Optical microtube with ultraviolet light protector caps. Reprinted with permission from.³⁹

Note: Reproduced from Harazim SM, Bolaños Quiñones VA, Kiravittaya S, Sanchez S, Schmidt OG. Lab-in-a-tube: on-chip integration of glass optofluidic ring resonators for label-free sensing applications. Lab Chip. 2012;12(15):2649,³⁹ with permission of the Royal Society of Chemistry. Available from http://pubs.rsc.org/en/Content/ArticleLanding/2012/LC/C2LC40275K#!divAbstract.
Abbreviation: CL, coupling link.

Figure 6 (A) Conceptual diagram of liquid core ring resonators (LCORR). (B) Cross-section of LCORR biosensor.

Abbreviation: WGM, whispering-gallery mode.

Sabaté del Río J, Steylaerts T, Henry OYF, et al. Real-time and label-free ring-resonator monitoring of solid-phase recombinase polymerase amplification. Biosens Bioelectron. 2015;73:130–137.

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Harazim SM, Bolaños Quiñones VA, Kiravittaya S, Sanchez S, Schmidt OG. Lab-in-a-tube:on-chip integration of glass optofluidic ring resonators for label-free sensing applications. Lab Chip. 2012;12(15):2649.

 Web of Science ®Google Scholar