Molecular and Serological Investigation of Infectious Bronchitis Virus in the East Shewa, Central Ethiopia

Figures & data

Figure 1 Map indicating study site prepared with GIS software.

Figure 2 Pooled swab sample and tissue Sample.

Table 1 Primers to Identify IBV Serotypes in Reverse Transcription Nested PCR

PCR types	Primer Designation	Sequence	Serotype	Fragment size (bp)	Reference
Reverse transcription PCR	XCE2+	5’-CACTGGTAATTTTTCAGATGG-3’	Universal	466 bp	[22]
	XCE2-	5’-CCTCTATAAACACCCTTGCA-3’
Reverse transcription Nested PCR	XCE3-	5’-CAGATTGCTTACAACCACC-3’	–	Universal
	DCE1+	5’-TTCCAATTATATCAAACCAGC-3’	D274	217 bp	[32]
	MCE1+	5’-AATACTACTTTTACGTTACAC-3’	Mass	295 bp
	BCE1+	5-AGTAGTTTTGTGTATAAACCA-3’	4/91	154 bp

Table 2 Infectious Bronchitis Seropositive Rates Based on Risk Factors

Variables	Category	Examined No.	No. Of Positive [%]	Odd Ratio	Confidence Interval	P- Value
Sex	Male	58	55[94.8]		0.055–2.922
	Female	138	137[99.2]	0.401		0.581
Age	Young	84	84[100]		0.931–0.999
	Adult	112	108[96.4]	0.964		0.137
Ventilation	Good	108	104[96.2]		0.947–0.999
	Poor	88	88[100]	0.973		0.574
Shoes Disinfection	Present	148	148[100]		1.002–1.188
	Absent	48	44[91.6]	1.091		0.003
Clinical Sign	Acute	19	19[100]
	Convalescent	177	173[97.7]	1.023	1.000–1.046	1.00
Farms	Adulala1	16	16[100]	0.228	0.228–0.228	0.097
	Adulala2	27	24[88.89]
	Dibayu	44	44[100]
	Girar	32	32[100]
	Gara Baru	32	32[100]
	Ada’a	21	21[100]
	Ada’a	24	23[95.8]
Purpose	Broliers	89	85[95.5]	2.824	0.00–0.288	0.084
	Layers	69	69[100]
	Breeders	38	38[100]
Over all prevalence		196	192[97.78]

Figure 3 Gross changes in chicken embryos caused by IBV at 15 days age, Dwarfing and curling of embryo at 15 days of age and also feather dystrophy.

Figure 4 IBV detected with general primer using RT-PCR.

Figure 5 IBV serotypes detected by species specific primers using nested PCR.

Jahantigh M, Salari S, Hedayati M. Detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens. Springer Plus. 2013;2(1):36. doi:10.1186/2193-1801-2-36

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