Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India

Figures & data

Table 1 Comparative results of serological tests and cultural isolation

Case no.	Case type	RBPT	SAT	Indirect ELISA	IgG ELISA	Blood culture
1	OE	–	1:80	–	–	ND
2	PUO	–	1:80	++	–	ND
3	OE	++	1:160	++	–	–
4	OE	–	1:80	++	–	ND
5	OE	–	1:80	++	–	ND
6	OE	–	1:160	++	–	–
7	PUO	–	1:80	++	–	ND
8	PUO	–	1:80	++	++	ND
9	PUO	–	1:80	–	–	ND
10	OE	++	1:160	++	–	–
11	OE	++	1:160	++	++	–
12	OE	++	1:160	++	++	–
13	PUO	++	1:160	–	++	–
14	OE	++	1:160	++	++	–
15	OE	–	1:80	++	++	ND
16	PUO	–	1:80	–	++	ND
17	OE	++	1:160	++	++	–
18	OE	++	1:160	++	++	–
19	OE	–	1:80	++	++	ND
20	OE	++	1:80	–	++	ND
21	OE	–	1:80	++	++	ND
22	OE	++	1:160	++	++	ND
23	OE	–	1:80	–	++	ND
24*	OE	++	1:80	–	–	+
25	PUO	++	1:80	–	–	ND

Note: –: negative; ++: positive; OE: occupationally exposed; PUO: pyrexia of unknown origin; ND: not done. *: Culture positive case.

Fig. 1 PCR amplification of the bcsp31 gene.

Lane 1: negative control; lane 2: positive control (Brucella abortus strain 19); lanes 3–6: Brucella isolate; M: 100 bp DNA ladder.

Fig. 2 PCR amplification of the IS711 gene.

Lane 1: negative control; lane 2: positive control (Brucella abortus strain 19); lane 3–6: Brucella isolate; M: 1.5 Kb DNA ladder.