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Abstract
The aim of this study was to elucidate the role of Found in Inflammatory Zone 1 (FIZZ1, also known as RELM-α or resistin-like molecule-α) in airway remodeling in asthma. We used a rat model of ovalbumin (OVA) sensitization and challenge to induce lung inflammation and remodeling. Expression of α -SMA in the lungs of OVA-treated rats was significantly elevated in the peribronchial regions compared with control saline-treated animals. Expression of FIZZ1 mRNA in alveolar epithelial type II cells (AECII) isolated from OVA-treated animals was higher than in control animals. Forced expression of recombinant FIZZ1 in rat-1 lung fibroblast cell line enhanced production of collagen type I and α -SMA compared with control transfected cells. These results suggest that FIZZ1 can induce fibroblasts to express markers of myofibroblast differentiation such as α -SMA and collagen type I, which are characteristic of early stages of airway remodeling seen in asthma.
| Abbreviations | ||
| FIZZ1 | = | found in inflammatory zone 1 |
| AECII | = | alveolar epithelial type II cell |
| α -SMA | = | α -smooth muscle actin |
| pEGFP-FIZZ1 | = | green fluorescent protein-FIZZ1 expressing plasmid |
| OVA | = | ovalbumin |
| AHR | = | hyperresponsiveness |
| RT-PCR | = | reverse transcription-polymerase chain reaction |
| ELISA | = | enzyme-linked immunoassay |
| SDS-PAGE | = | sodium dodecyl sulfate polyacrylamide gel electrophoresis |
| HE | = | hematoxylin-eosin |
| IDV | = | Integrated Density Value |
| IOD | = | Integrated Optical Density |
| PI3K | = | phosphatidylinositol 3-kinase |
| DMEM | = | Dulbecco's Modified Eagle's Medium |
| Abbreviations | ||
| FIZZ1 | = | found in inflammatory zone 1 |
| AECII | = | alveolar epithelial type II cell |
| α -SMA | = | α -smooth muscle actin |
| pEGFP-FIZZ1 | = | green fluorescent protein-FIZZ1 expressing plasmid |
| OVA | = | ovalbumin |
| AHR | = | hyperresponsiveness |
| RT-PCR | = | reverse transcription-polymerase chain reaction |
| ELISA | = | enzyme-linked immunoassay |
| SDS-PAGE | = | sodium dodecyl sulfate polyacrylamide gel electrophoresis |
| HE | = | hematoxylin-eosin |
| IDV | = | Integrated Density Value |
| IOD | = | Integrated Optical Density |
| PI3K | = | phosphatidylinositol 3-kinase |
| DMEM | = | Dulbecco's Modified Eagle's Medium |