Deleterious effects of incense smoke exposure on kidney function and architecture in male albino rats

Abstract

Context: Previous studies, including ours, have shown adverse effects of incense smoke on human health. However, the effect of incense smoke on kidney function and structure remains unknown.

Objective: To evaluate possible adverse effects of incense smoke on kidney function and architecture in albino rats after chronic exposure to Arabian incense.

Materials and methods: Emission characteristics including particle size distribution, volatile organic compounds (VOCs) and polycyclic aromatic hydrocarbons (PAHs) were determined by gravimetric and GCMS analyses. Kidney functional markers, oxidative stress and inflammatory markers were measured by standard or ELISA based procedures. Ultrastructural changes in kidney were examined by transmission electron microscope (TEM) and the gene expression of xenobiotic metabolizing enzymes including cytochrome P-450-1A1 (CYP1A1) and CYP1A2 were studied by real time PCR.

Results: Rats exposed to incense smoke demonstrated a significant increase in serum creatinine, uric acid, blood urea nitrogen (BUN), tissue malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α) and interleukin-4 (IL-4) levels and a significant decline in tissue reduced glutathione (GSH) and catalase activity. Incense smoke exposed rats also displayed marked ultrastructural changes in kidney tissue. Further, a significant increase in tissue gene expression of both CYP1A1 and CYP1A2 was noted in exposed rats.

Discussion: Changes to kidney functional markers and architecture appear to be mediated through augmented oxidative stress and inflammation.

Conclusion: Long-term exposure to incense smoke may have deleterious effects on kidney function and architecture. Though, inhalation is the rout of exposure, findings of this study underscore that incense smoke may also have an effect on non-pulmonary tissues.

• Incense smoke
• inflammation
• kidney
• kidney architecture
• oxidative stress
• xenobiotic metabolizing enzymes

Acknowledgments

We acknowledge the technical help of Mohammed Arshad.

Declaration of interest

Authors report no declarations of interest. This study was supported by the Research Chair for Biomedical Applications of Nanomaterials, Deanship of Scientific Research, King Saud University, Riyadh, Saudi Arabia.