Effects of Zn²⁺ and Mn²⁺ on the photo-fermentative performance of HY01 in biohydrogen production from xylose fermentation

ABSTRACT

The addition of specific ions has a significant effect on the characteristics and kinetics of hydrogen production by photofermentation (PFHP). In this article, a new basic research investigated by us find that adjusting the addition ratio of Zn²⁺ and Mn²⁺ ions in the R. sphaeroides HY01 system can effectively improve their biohydrogen production from xylose. The largest biohydrogen yield can be obtained as high as 219.2 mL/g xylose at concentration levels of 12 mg/L Zn²⁺ and 8 mg/L Mn²⁺. The shortest lag time is 5.76 h, along with most of the organic energy in the substrate transferred to H₂. To evaluate the effect of Zn²⁺ and Mn²⁺, the Gompertz model with appropriately modification is applied on the photosynthetic bacteria R. sphaeroides HY01, and the corresponding constants are obtained by fitting the model, which is of great guiding significance for the optimization of fermentation conditions.

KEYWORDS:

• Manganese ion
• zinc ion
• BioH₂ production
• metabolic pathway
• photo fermentation

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethical approval

Any studies with human participants or animals were not contained in this article.

Author contributions

Yuheng Zhou: Supervision, Writing – review & editing, Methodology

Xiaohui Wang: Methodology, Data curation, Writing – original draft.

Xubo Huang: Data curation, Writing – original draft.

Hui Deng: Funding acquisition, Conceptualization.

Yuntao Hu: Characterizations support, Writing – review & editing.

Data availability statement

The data generated and analyzed during the current study are available from the corresponding author on reasonable request.

Additional information

Funding

This work was funded by the Science and Technology Project of China Petrochemical Corporation (P15163, P19029-5 and P19902) and Zhejiang Provincial Natural Science Foundation of China.